Chicken

Genome-wide association study of meat quality traits in chicken

T. Zhang, Fan, Q. C., Wang, J. Y., Zhang, G. X., Gu, Y. P., and Tang, Y., “Genome-wide association study of meat quality traits in chicken”, vol. 14, pp. 10452-10460, 2015.

Meat quality traits are very important in the poultry industry. To identify single nucleotide polymorphisms (SNPs) and candidate genes affecting meat quality traits, a genome-wide association study was performed using the Illumina chicken 60K SNP beadchip in Jinghai yellow chicken. Four meat quality traits were measured. Two SNPs reached 5% Bonferroni genome-wide significance (P < 1.8E-6) and 7 SNPs reached “suggestive” genome-wide significance (P < 3.59E-6) with meat quality.

Identification of single nucleotide polymorphisms in the ASB15 gene and their associations with chicken growth and carcass traits

Y. C. Wang, Jiang, R. R., Kang, X. T., Li, Z. J., Han, R. L., Geng, J., Fu, J. X., Wang, J. F., and Wu, J. P., “Identification of single nucleotide polymorphisms in the ASB15 gene and their associations with chicken growth and carcass traits”, vol. 14, pp. 11377-11388, 2015.

ASB15 is a member of the ankyrin repeat and suppressor of cytokine signaling box family, and is predominantly expressed in skeletal muscle. In the present study, an F2 resource population of Gushi chickens crossed with Anka broilers was used to investigate the genetic effects of the chicken ASB15 gene. Two single nucleotide polymorphisms (SNPs) (rs315759231 A>G and rs312619270 T>C) were identified in exon 7 of the ASB15 gene using forced chain reaction-restriction fragment length polymorphism and DNA sequencing.

Construction of the mammalian expressing vector pEGFP-N1-P53 and its expression successful in chicken fibroblast cells and blastoderm

Z. Song, Li, Z. H., Lei, X. Q., Xu, T. S., Zhang, X. H., Li, Y. J., Zhang, G. M., Xi, S. M., Yang, Y. B., and Wei, Z. G., “Construction of the mammalian expressing vector pEGFP-N1-P53 and its expression successful in chicken fibroblast cells and blastoderm”, vol. 14, pp. 931-939, 2015.

The enhanced green fluorescent protein (EGFP) pEGFP-N1-P53 eukaryotic expression vector, which contains the human tumor suppressor p53, was constructed and transfected into chicken fibroblast cells and stage-X blastoderm to analyze the transfection efficiency. The complementary DNA of the human p53 gene was cloned by reverse transcription-polymerase chain reaction from human peripheral blood and inserted into the pEGFP-N1 vector by HindIII and BamHI double digestion.

Confined housing system increased abdominal and subcutaneous fat deposition and gene expressions of carbohydrate response element-binding protein and sterol regulatory element-binding protein 1 in chicken

Q. Li, Zhao, X. L., Gilbert, E. R., Liu, Y. P., Wang, Y., Qiu, M. H., and Zhu, Q., “Confined housing system increased abdominal and subcutaneous fat deposition and gene expressions of carbohydrate response element-binding protein and sterol regulatory element-binding protein 1 in chicken”, vol. 14, pp. 1220-1228, 2015.

Free-range production system is increasingly being used in poultry breeding and feed production in many countries. The objective of the current experiment was to evaluate the effects of different raising systems on fat-related traits and mRNA levels of liver lipogenesis genes in Erlang Mountainous chicken.

Analysis of genetic diversity of the heat shock protein 70 gene on the basis of abundant sequence polymorphisms in chicken breeds

J. K. Gan, Jiang, L. Y., Kong, L. N., Zhang, X. Q., and Luo, Q. B., “Analysis of genetic diversity of the heat shock protein 70 gene on the basis of abundant sequence polymorphisms in chicken breeds”, vol. 14, pp. 1538-1545, 2015.

This study was designed to detect the sequence variation of the chicken heat shock protein 70 (HSP70) gene. A total of 102 individuals from 8 native Chinese breeds together with Dwarf White Chicken and Red Junglefowl were used to detect sequence variations. The coding regions of the chicken HSP70 gene from 102 individuals were cloned and sequenced. Thirty-six variations were identified, which included 34 single nucleotide polymorphisms and 2 indel mutations. Fifty-seven haplotypes were observed, of which, 43 were breed-specific and 14 were shared.

Analysis of the genetic effects of CAPN1 gene polymorphisms on chicken meat tenderness

J. T. Shu, Zhang, M., Shan, Y. J., Xu, W. J., Chen, K. W., and Li, H. F., “Analysis of the genetic effects of CAPN1 gene polymorphisms on chicken meat tenderness”, vol. 14, pp. 1393-1403, 2015.

The micromolar calcium-activated neutral protease gene (CAPN1) is a physiological candidate gene for meat tenderness. Four previously identified single nucleotide polymorphism (SNP) markers located within the CAPN1 gene were evaluated for their associations with variation in the meat tenderness of a Chinese indigenous chicken breed, a higher meat quality breed (i.e., Qingyuan partridge chicken), and the commercial Recessive White chicken breed.

Microarray analysis of differentially expressed genes in the liver between Bai’er layers and broilers

Q. - G. Wang, Zhang, H. - F., Wang, S. - Z., Gao, G. L., Leng, L., Na, W., and Li, H., “Microarray analysis of differentially expressed genes in the liver between Bai’er layers and broilers”, vol. 14, pp. 2885-2889, 2015.

In this study, we profiled gene expression in chicken liver and screened differentially expressed genes in the Bai’er layers and fat line broilers. Birds were derived from the 14th generation of Northeast Agricultural University fat broiler lines and Bai’er layers. Chicken genome arrays were used to screen differentially expressed genes in liver tissue from the Bai’er layers and fat line broilers. We screened 671 differentially expressed genes between broilers and layers at the ages of 2 and 4 weeks.

Molecular characterization, expression, and functional analysis of NOD1 in Qingyuan partridge chicken

Z. Y. Tao, Zhu, C. H., Shi, Z. H., Song, C., Xu, W. J., Song, W. T., Zou, J. M., and Qin, A. J., “Molecular characterization, expression, and functional analysis of NOD1 in Qingyuan partridge chicken”, vol. 14, pp. 2691-2701, 2015.

Nucleotide-binding oligomerization domain-containing protein-1 (NOD1) is a cytoplasmic pattern recognition receptor (PRR) and a key member of the NOD-like receptor (NLR) family. It has been reported that NLRs recognize a variety of microbial infections to induce the host innate immune response via modulation of NF-κB signaling. However, no reports on chicken NOD1 have been reported to date. In the current study, the full-length cDNA sequence of NOD1 was cloned. The complete open reading frame of NOD1 contains 2856 bp and encodes a 951 amino acid protein.

Molecular cloning and tissue distribution profiles of the chicken R-spondin1 gene

Y. Q. Han, Geng, J., Shi, H. T., Zhang, X. M., Du, L. L., Liu, F. T., Li, M. M., Wang, X. T., Wang, Y. Y., and Yang, G. Y., “Molecular cloning and tissue distribution profiles of the chicken R-spondin1 gene”, vol. 14, pp. 3090-3097, 2015.

Rspo1 belongs to the Rspo family, which is composed of 4 members (Rspo1-4) that share 40 to 60% sequence homology and similar domain organizations, and regulate the WNT signaling pathway via a common mechanism. Rspo1 plays a key role in vertebrate development and is an effective mitogenic factor of gastrointestinal epithelial cells. We report the cloning of chicken Rspo1 and its gene expression distribution among tissues.

Expression of β-defensins in intestines of chickens injected with vitamin D3 and lipopolysaccharide

L. Lu, Li, S. M., Zhang, L., Liu, X. Q., Li, D. Y., Zhao, X. L., and Liu, Y. P., “Expression of β-defensins in intestines of chickens injected with vitamin D3 and lipopolysaccharide”, vol. 14, pp. 3330-3337, 2015.

The objective of this study was to evaluate the effect of vitamin D3 (VD3) on the regulation of chicken intestinal β-defensin genes under normal and lipopolysaccharides (LPS) conditions. Four treatment groups were used, including a negative control group, VD3-injection group, LPS-injection group, and both VD3-injection and LPS-injection group.

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