Chicken embryo fibroblasts

Construction of the mammalian expressing vector pEGFP-N1-P53 and its expression successful in chicken fibroblast cells and blastoderm

Z. Song, Li, Z. H., Lei, X. Q., Xu, T. S., Zhang, X. H., Li, Y. J., Zhang, G. M., Xi, S. M., Yang, Y. B., and Wei, Z. G., Construction of the mammalian expressing vector pEGFP-N1-P53 and its expression successful in chicken fibroblast cells and blastoderm, vol. 14, pp. 931-939, 2015.

The enhanced green fluorescent protein (EGFP) pEGFP-N1-P53 eukaryotic expression vector, which contains the human tumor suppressor p53, was constructed and transfected into chicken fibroblast cells and stage-X blastoderm to analyze the transfection efficiency. The complementary DNA of the human p53 gene was cloned by reverse transcription-polymerase chain reaction from human peripheral blood and inserted into the pEGFP-N1 vector by HindIII and BamHI double digestion.

Subscribe to Chicken embryo fibroblasts