Chromobacterium violaceum

Superoxide radical-generating compounds activate a predicted promoter site for paraquat-inducible genes of the Chromobacterium violaceum bacterium in a dose-dependent manner

J. E. Gabriel, Guerra-Slompo, E. P., de Souza, E. M., de Carvalho, F. A. L., Madeira, H. M. F., and de Vasconcelos, A. T. R., Superoxide radical-generating compounds activate a predicted promoter site for paraquat-inducible genes of the Chromobacterium violaceum bacterium in a dose-dependent manner, vol. 14, pp. 10139-10144, 2015.

The purpose of the present study was to functionally evaluate the influence of superoxide radical-generating compounds on the heterologous induction of a predicted promoter region of open reading frames for paraquat-inducible genes (pqi genes) revealed during genome annotation analyses of the Chromobacterium violaceum bacterium. A 388-bp fragment corresponding to a pqi gene promoter of C. violaceum was amplified using specific primers and cloned into a conjugative vector containing the Escherichia coli lacZ gene without a promoter.

Anthranilate synthase subunit organization in Chromobacterium violaceum

C. A. Carminatti, Oliveira, I. L., Recouvreux, D. O. S., Antônio, R. V., and Porto, L. M., Anthranilate synthase subunit organization in Chromobacterium violaceum, vol. 7, pp. 830-838, 2008.

Tryptophan is an aromatic amino acid used for protein synthesis and cellular growth. Chromobacterium violaceum ATCC 12472 uses two tryptophan molecules to synthesize violacein, a secondary metabolite of pharmacological interest. The genome analysis of this bacterium revealed that the genes trpA-F and pabA-B encode the enzymes of the tryptophan pathway in which the first reaction is the conversion of chorismate to anthranilate by anthranilate synthase (AS), an enzyme complex. In the present study, the organization and structure of AS protein subunits from C.

Identification of Chromobacterium violaceum genes with potential biotechnological application in environmental detoxification

M. S. P. Carepo, de Azevedo, J. S. Nina, Porto, J. I. R., Bentes-Sousa, A. R., Batista, Jda Silva, da Silva, A. L. C., and Schneider, M. P. C., Identification of Chromobacterium violaceum genes with potential biotechnological application in environmental detoxification, vol. 3, pp. 181-194, 2004.

Chromobacterium violaceum is a Gram-negative bacterium found in a wide variety of tropical and subtropical ecosystems. The complete genome sequence of C. violaceum ATCC 12472 is now available, and it has considerable biotechnological potential for various applications, such as environmental detoxification, as well as medical and agricultural use. We examined the biotechnological potential of C. violaceum for environmental detoxification.

Energetic metabolism of Chromobacterium violaceum

T. B. Creczynski-Pasa and Antônio, R. V., Energetic metabolism of Chromobacterium violaceum, vol. 3, pp. 162-166, 2004.

Chromobacterium violaceum is a free-living microorganism, normally exposed to diverse environmental conditions; it has a versatile energy-generating metabolism. This bacterium is capable of exploiting a wide range of energy resources by using appropriate oxidases and reductases. This allows C. violaceum to live in both aerobic and anaerobic conditions. In aerobic conditions, C.

Chemotaxis and flagellar genes of Chromobacterium violaceum

M. Pereira, Parente, J. Alves, Bataus, L. Artur Mend, Cardoso, Ddas Dores, Soares, R. Bastos Asc, and Soares, C. Maria de A., Chemotaxis and flagellar genes of Chromobacterium violaceum, vol. 3, pp. 92-101, 2004.

The availability of the complete genome of the Gram-negative β-proteobacterium Chromobacterium violaceum has increasingly impacted our understanding of this microorganism. This review focuses on the genomic organization and structural analysis of the deduced proteins of the chemosensory adaptation system of C. violaceum. C. violaceum has multiple homologues of most chemotaxis genes, organized mostly in clusters in the bacterial genome. We found at least 67 genes, distributed in 10 gene clusters, involved in the chemotaxis of C. violaceum.

Genetic analysis of violacein biosynthesis by Chromobacterium violaceum

R. Vasconcell Antônio and Creczynski-Pasa, T. B., Genetic analysis of violacein biosynthesis by Chromobacterium violaceum, vol. 3, pp. 85-91, 2004.

Chromobacterium violaceum presents a distinctive phenotypic characteristic, the production of a deep violet pigment named violacein. Although the physiological function of this pigment is not well understood, the sequencing of the genome of this bacterium has given some insight into the mechanisms and control of violacein production. It was found that erythrose-4-phosphate (E4P), a precursor to aromatic amino acid biosynthesis, is produced by the non-oxidative portion of the hexose monophosphate pathway, since it lacks 6-phosphogluconate dehydrogenase.

Bacteriophages and insertion sequences of Chromobacterium violaceum ATCC 12472

R. de Almeida, Trevilato, P. Beltramini, Bartoleti, L. Aparecida, Proença-Módena, J. Luiz, Hanna, E. Seixas, Gregoracci, G. Bueno, and Brocchi, M., Bacteriophages and insertion sequences of Chromobacterium violaceum ATCC 12472, vol. 3, pp. 76-84, 2004.

A fluid genome is a great advantage to prokaryotes, enabling quick adaptation to various types of ecological niches and to diverse environmental selective pressures. A substantial portion of these sudden changes is mediated by lateral gene transfer (LGT), through genetic recombination mechanisms, such as transformation, conjugation and transduction. The recent sequencing of several organisms has offered a new approach to the study of LGT, using comparison and analysis of nucleotide sequences dispersed throughout the genome of these species.

Gene expression in Chromobacterium violaceum

R. Silva, Araripe, J. R., Rondinelli, E., and Ürményi, T. P., Gene expression in Chromobacterium violaceum, vol. 3, pp. 64-75, 2004.

The repertoire of 4,431 open reading frames (ORFs), eight rRNA operons and 98 tRNA genes of Chromobacterium violaceum must be expressed in a regulated manner for successful adaptation to a wide variety of environmental conditions. To accomplish this feat, the organism relies on protein machineries involved in transcription, RNA processing and translation. Analysis of the C.

DNA repair in Chromobacterium violaceum

F. Teixeira Duarte, de Carvalho, F. Marques, Silva, U. Bezerra e, Scortecci, K. Castanho, Blaha, C. Alfredo Ga, Agnez-Lima, L. Fassarella, and de Medeiros, S. Regina Bat, DNA repair in Chromobacterium violaceum, vol. 3, pp. 167-180, 2004.

Chromobacterium violaceum is a Gram-negative β-proteobacterium that inhabits a variety of ecosystems in tropical and subtropical regions, including the water and banks of the Negro River in the Brazilian Amazon. This bacterium has been the subject of extensive study over the last three decades, due to its biotechnological properties, including the characteristic violacein pigment, which has antimicrobial and anti-tumoral activities. C.

Chromobacterium violaceum genome: molecular mechanisms associated with pathogenicity

C. F. Alves de Brito, Carvalho, C. M. B., Santos, F. R., Gazzinelli, R. T., Oliveira, S. C., Azevedo, V., and Teixeira, S. M. R., Chromobacterium violaceum genome: molecular mechanisms associated with pathogenicity, vol. 3, pp. 148-161, 2004.

Chromobacterium violaceum is a versatile, Gram-negative β-protebacterium that grows in a variety of ecosystems in tropical and subtropical areas, such as the water and borders of the Negro River, in the Amazon region of Brazil. Although it is a saprophyte and is generally considered non-pathogenic, sporadic cases of human infection have been described, mainly in young children and in immunodeficient individuals. Although rare, infections with C. violaceum are characterized by rapid dissemination and high mortality. With the complete genome sequence of C.

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