Cloning

Cloning and expression analysis of pepper chlorophyll catabolite reductase gene CaRCCR

H. - J. Xiao, Jin, J. - H., Chai, W. - G., and Gong, Z. - H., Cloning and expression analysis of pepper chlorophyll catabolite reductase gene CaRCCR, vol. 14, pp. 368-379, 2015.

Opening the porphyrin macrocycle of pheophorbide a and forming the primary fluorescent chlorophyll catabolites are key steps in the chlorophyll catabolism pathway. These steps are catalyzed by pheophorbide a oxygenase and red chlorophyll catabolite reductase (RCCR). In this study, a novel RCCR gene, CaRCCR, was isolated from the pepper (Capsicum annuum L.). The full-length CaRCCR complementary DNA is comprised of 1173 bp, contains an open reading frame of 945 bp, and encodes a 314-amino acid protein.

A C-type lectin fold gene from Japanese scallop Mizuhopecten yessoensis, involved with immunity and metamorphosis

X. B. Bao, He, C. B., Fu, C. D., Wang, B., Zhao, X. M., Gao, X. G., and Liu, W. D., A C-type lectin fold gene from Japanese scallop Mizuhopecten yessoensis, involved with immunity and metamorphosis, vol. 14, pp. 2253-2267, 2015.

C-type lectins are a superfamily of Ca2+-dependent carbohydrate-recognition proteins that are well known for their participation in pathogen recognition and clearance. In this study, a putative C-type lectin fold (MyCLF) gene was identified from the Japanese scallop Mizuhopecten yessoensis. The full-length of MyCLF was 645 bp, encoding a polypeptide of 167 amino acids.

Molecular cloning and characterization, and prokaryotic expression of the GnRH1 gene obtained from Jinghai yellow chicken

T. Zhang, Zhang, G. X., Han, K. P., Tang, Y., Wang, J. Y., Fan, Q. C., Chen, X. S., Wei, Y., and Wang, Y. J., Molecular cloning and characterization, and prokaryotic expression of the GnRH1 gene obtained from Jinghai yellow chicken, vol. 14, pp. 2831-2849, 2015.

The gonadotropin-releasing hormone (GnRH) plays an important role in the control of reproductive functions. Recent studies have reported the occurrence of GnRH molecular variants in numerous species. In this study, the GnRH1 gene from Jinghai yellow chicken was cloned by reverse transcriptase-polymerase chain reaction and transformed into BL21 (DE3) competent cells. The GnRH1 gene and amino acid sequences were subjected to bioinformatic analyses.

Molecular cloning and tissue distribution profiles of the chicken R-spondin1 gene

Y. Q. Han, Geng, J., Shi, H. T., Zhang, X. M., Du, L. L., Liu, F. T., Li, M. M., Wang, X. T., Wang, Y. Y., and Yang, G. Y., Molecular cloning and tissue distribution profiles of the chicken R-spondin1 gene, vol. 14, pp. 3090-3097, 2015.

Rspo1 belongs to the Rspo family, which is composed of 4 members (Rspo1-4) that share 40 to 60% sequence homology and similar domain organizations, and regulate the WNT signaling pathway via a common mechanism. Rspo1 plays a key role in vertebrate development and is an effective mitogenic factor of gastrointestinal epithelial cells. We report the cloning of chicken Rspo1 and its gene expression distribution among tissues.

Molecular cloning and expression analysis of TRAF3 in chicken

H. L. Yang, Feng, Z. Q., Zeng, S. Q., Li, S. M., Zhu, Q., and Liu, Y. P., Molecular cloning and expression analysis of TRAF3 in chicken, vol. 14, pp. 4408-4419, 2015.

Tumor necrosis factor receptor-associated factor 3 (TRAF3) is a crucial regulator that suppresses c-Jun N-terminal kinase and non-canonical nuclear factor-kB signaling, but facilitates type I interferon production. To determine TRAF3 function in innate immune responses among birds, particularly chicken, we cloned and characterized the chicken TRAF3 gene (chTRAF3) and detected its tissue expression profile in chicken.

Molecular cloning and bioinformatic analysis of the Streptococcus agalactiae neuA gene isolated from tilapia

E. L. Wang, Wang, K. Y., Chen, D. F., Geng, Y., Huang, L. Y., Wang, J., and He, Y., Molecular cloning and bioinformatic analysis of the Streptococcus agalactiae neuA gene isolated from tilapia, vol. 14, pp. 6003-6017, 2015.

Cytidine monophosphate (CMP) N-acetylneuraminic acid (NeuNAc) synthetase, which is encoded by the neuA gene, can catalyze the activation of sialic acid with CMP, and plays an important role in Streptococcus agalactiae infection pathogenesis. To study the structure and function of the S. agalactiae neuA gene, we isolated it from diseased tilapia, amplified it using polymerase chain reaction (PCR) with specific primers, and cloned it into a pMD19-T vector.

Comparative analysis and molecular characterization of genomic sequences and proteins of FABP4 and FABP5 from the giant panda (Ailuropoda melanoleuca)

B. Song, Hou, Y. L., Ding, X., Wang, T., Wang, F., Zhong, J. C., Xu, T., Zhong, J., Hou, W. R., and Shuai, S. R., Comparative analysis and molecular characterization of genomic sequences and proteins of FABP4 and FABP5 from the giant panda (Ailuropoda melanoleuca), vol. 13, pp. 992-1004, 2014.

Fatty acid binding proteins (FABPs) are a family of small, highly conserved cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. In this study, cDNA and genomic sequences of FABP4 and FABP5 were cloned successfully from the giant panda (Ailuropoda melanoleuca) using reverse transcription polymerase chain reaction (RT-PCR) technology and touchdown-PCR.

Molecular cloning and characterization of the pseudorabies virus UL31 gene

M. L. Li, Zhao, Z. Y., Cui, W., Mo, C. C., Wang, J. L., and Cai, M. S., Molecular cloning and characterization of the pseudorabies virus UL31 gene, vol. 13, pp. 1832-1847, 2014.

We amplified a 816-bp sequence of the UL31 gene from the pseudorabies virus (PRV) Becker strain genome. Evidence that this was the UL31 gene was confirmed by cloning and sequencing. The PRV UL31 gene encodes a putative protein of 271-amino acid residues, which was designated the UL31 protein. Bioinformatic analysis indicated that PRV UL31 contains a conserved PHA03328 domain, closely related with the herpes virus nuclear egress lamina protein UL31 family and highly conserved among counterparts encoded by herpes UL31 genes.

Identification of spliced mRNA isoforms of retinoid X receptor (RXR) in the Oriental freshwater prawn Macrobrachium nipponense

Z. Li, Wang, W. Q., Zhang, E. F., and Qiu, G. F., Identification of spliced mRNA isoforms of retinoid X receptor (RXR) in the Oriental freshwater prawn Macrobrachium nipponense, vol. 13, pp. 3914-3926, 2014.

Retinoid X receptors (RXR) are members of the nuclear receptor family that are conserved from invertebrates to vertebrates, and they play an essential role in regulating reproductive maturation, molting, and embryo development. In this study, five RXR isoforms, named RXRL2 (L, long form), RXRL3, RXRS1 (S, short form), RXRS2, and RXRS3, containing six domains from A to F, were cloned from the prawn Macrobrachium nipponense using 5ꞌ- and 3ꞌ- rapid amplification of cDNA ends.

Identification and authentication of Rosa species through development of species-specific SCAR marker(s)

K. M. I. Bashir, Awan, F. S., Khan, I. A., Khan, A. I., and Usman, M., Identification and authentication of Rosa species through development of species-specific SCAR marker(s), vol. 13, pp. 4130-4139, 2014.

Roses (Rosa indica) belong to one of the most crucial groups of plants in the floriculture industry. Rosa species have special fragrances of interest to the perfume and pharmaceutical industries. The genetic diversity of plants based on morphological characteristics is difficult to measure under natural conditions due to the influence of environmental factors, which is why a reliable fingerprinting method was developed to overcome this problem. The development of molecular markers will enable the identification of Rosa species.

Pages

Subscribe to Cloning