Cloning

Molecular cloning, tissue expression pattern, and copy number variation of porcine SCUBE3

X. Liu, Wang, L. G., Zhang, L. C., Yan, H., Zhao, K. B., Liang, J., Li, N., Pu, L., Zhang, T., Wang, L. X., Liu, X., Wang, L. G., Zhang, L. C., Yan, H., Zhao, K. B., Liang, J., Li, N., Pu, L., Zhang, T., and Wang, L. X., “Molecular cloning, tissue expression pattern, and copy number variation of porcine SCUBE3”, vol. 15, p. -, 2016.

The signal peptide CUB EGF-like domain-containing protein 3 (SCUBE3) gene is a member of SCUBE gene family and plays important roles in bone cell biology and the determination of limb bone length. In this study, the full-length transcript of porcine SCUBE3 was cloned using reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends. The full-length sequence of porcine SCUBE3 cDNA was 4131 base pairs and included 21 exons.

Molecular cloning and expression analysis of MAT1 gene in black tiger shrimp (Penaeus monodon)

Y. Wang, Fu, M. J., Zhao, C., Bao, W. Y., Zhou, F. L., Yang, Q. B., Jiang, S. G., Qiu, L. H., Wang, Y., Fu, M. J., Zhao, C., Bao, W. Y., Zhou, F. L., Yang, Q. B., Jiang, S. G., and Qiu, L. H., “Molecular cloning and expression analysis of MAT1 gene in black tiger shrimp (Penaeus monodon)”, vol. 15, p. -, 2016.

MAT1 (ménage à trois 1), an assembly factor and targeting subunit of the CDK-dependent kinase (CAK), can regulate the cell cycle, transcription, and DNA repair. This study was intended to investigate the role of MAT1 in the reproductive maturation of black tiger shrimp (Penaeus monodon). In this study, the P. monodon MAT1 (PmMAT1) gene was identified and characterized. The full-length cDNA of PmMAT1 was 1490 bp in length with an open-reading frame of 993 bp corresponding to 330 amino acids.

Molecular characterization and tissue expression profile of porcine Ephrin-B2

D. Xu, Ma, H. M., Xu, D., and Ma, H. M., “Molecular characterization and tissue expression profile of porcine Ephrin-B2”, vol. 15, p. -, 2016.

Ephrin-B2 (EFNB2) is a signaling molecule that plays an important role in cell adhesion, proliferation, and migration in humans. However, little is known about this molecule in pigs. In order to investigate whether EFNB2 is associated with the skeletal muscle in pigs, we cloned the full-length cDNA of EFNB2 (GenBank accession No. KF500033) from the longissimus dorsi muscle of Yorkshire pigs by rapid amplification of cDNA ends.

Characterization of allele-specific expression of the X-linked gene MAO-A in trophectoderm cells of bovine embryos produced by somatic cell nuclear transfer

A. R. Ferreira, Filho, L. F. C. Aguiar, Sousa, R. V., Sartori, R., and Franco, M. M., “Characterization of allele-specific expression of the X-linked gene MAO-A in trophectoderm cells of bovine embryos produced by somatic cell nuclear transfer”, vol. 14, pp. 12128-12136, 2015.

Somatic cell nuclear transfer (SCNT) may affect epigenetic mechanisms and alter the expression of genes related to embryo development and X chromosome inactivation (XCI). We characterized allele-specific expression of the X-linked gene monoamine oxidase type A (MAO-A) in the trophectoderm (TF) of embryos produced by SCNT. Total RNA was isolated from individual biopsies (N = 25), and the allele-specific expression assessed by reverse transcription-polymerase chain reaction-restriction fragment length polymorphism.

Cloning, identification, and bioinformatics analysis of a putative aquaporin TsAQP from Trichinella spiralis

J. M. Cui, Zhang, N. Z., Li, W. H., Yan, H. B., and Fu, B. Q., “Cloning, identification, and bioinformatics analysis of a putative aquaporin TsAQP from Trichinella spiralis”, vol. 14, pp. 12699-12709, 2015.

Vaccination as a preventative strategy against Trichinella spiralis infection is an ongoing effort, although no ideal vaccine candidates have been identified until now. Identification of more effective antigens that have a role in essential life stages of the parasite and that may be effective vaccine candidates is therefore of importance. In the present study, we identified a novel aquaporin gene (TsAQP) from T. spiralis, and the potential antigenicity of TsAQP was evaluated by epitope prediction.

Molecular characterization and expression patterns of insulin-like growth factor-binding protein genes in postnatal Nanjiang brown goats

S. Y. Zhan, Chen, L., Li, L., Wang, L. J., Zhong, T., and Zhang, H. P., “Molecular characterization and expression patterns of insulin-like growth factor-binding protein genes in postnatal Nanjiang brown goats”, vol. 14, pp. 12547-12560, 2015.

Insulin-like growth factor-binding proteins (IGFBPs) play a key role in modulating insulin-like growth factors (IGFs), and are considered candidate genes for growth traits in livestock. In this study, we identified the complete coding sequences of IGFBP-1 to -6 in the Nanjiang brown goat, and assessed gene tissue expression patterns by quantitative polymerase chain reaction. Expression of mRNA for the six gene targets was detectable in liver, heart, and longissimus dorsi (LD) muscle.

Analysis of an “off-ladder” allele at the Penta D short tandem repeat locus

Y. L. Yang, Wang, J. G., Wang, D. X., Zhang, W. Y., Liu, X. J., Cao, J., and Yang, S. L., “Analysis of an “off-ladder” allele at the Penta D short tandem repeat locus”, vol. 14, pp. 15096-15101, 2015.

Kinship testing of a father and his son from Guangxi, China, the location of the Zhuang minority people, was performed using the PowerPlex® 18D System with a short tandem repeat typing kit. The results indicated that both the father and his son had an off-ladder allele at the Penta D locus, with a genetic size larger than that of the maximal standard allelic ladder. To further identify this locus, monogenic amplification, gene cloning, and genetic sequencing were performed.

Molecular characterization, tissue expression profile, and SNP analysis of porcine SLC13A5

L. Y. Wang, Jiang, J., and Ma, H. M., “Molecular characterization, tissue expression profile, and SNP analysis of porcine SLC13A5”, vol. 14, pp. 16090-16101, 2015.

Solute carrier family 13 (sodium-dependent citrate transporter member 5, SLC13A5) gene has been recently found to play an important role in intramuscular fat content in pigs. In this study, the full-length cDNA of porcine SLC13A5 was obtained from the longissimus dorsi muscle of Shaziling pigs, using the rapid amplification of cDNA ends technique. Full-length porcine SLC13A5 cDNA was 2118 bp, with a 1665-bp open reading frame encoding 554 amino acids.

Cloning, characterization, and expression of cadmium-induced metallothionein-2 gene from earthworm Pheretima aspergillum (E. Perrier)

L. Gong, Li, W., Li, J., Li, W. E., Wu, W. R., and Yu, L. W., “Cloning, characterization, and expression of cadmium-induced metallothionein-2 gene from earthworm Pheretima aspergillum (E. Perrier)”, vol. 14, pp. 16782-16792, 2015.

Metallothioneins (MTs) are ubiquitous metal-binding, cysteine-rich proteins, associated with metal accumulation and thus providing protection against toxic heavy metals such as cadmium (Cd). To investigate the mechanisms of enrichment of Cd in the earthworm Pheretima aspergillum, we isolated and cloned metallothionein-2 (MT-2) cDNA (538 bp) from P. aspergillum, analyzed its sequence, and examined MT-2 transcription levels by relative quantitative real-time PCR under different concentrations of Cd. The sequence of P.

Molecular cloning and expression analysis of a pearl oyster (Pinctada martensii) heat shock protein 90 (HSP90)

H. Y. Liang, Wang, Z. X., Lei, Q. N., Huang, R. L., Deng, Y. W., Wang, Q. H., Jiao, Y., and Du, X. D., “Molecular cloning and expression analysis of a pearl oyster (Pinctada martensii) heat shock protein 90 (HSP90)”, vol. 14, pp. 18778-18791, 2015.

Heat shock protein 90 (HSP90) is an important molecular chaperone required for proper folding of cellular proteins, and thus, it plays an essential role in protecting cells from damage during stress. In this study, an HSP90 cDNA designated PmHSP90 was cloned from the mantle tissue of the pearl oyster Pinctada martensii using reverse transcription polymerase chain reaction (RT-PCR) coupled with the rapid amplification of cDNA ends (RACE) approach.

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