Cloning

Characterization of allele-specific expression of the X-linked gene MAO-A in trophectoderm cells of bovine embryos produced by somatic cell nuclear transfer

A. R. Ferreira, Filho, L. F. C. Aguiar, Sousa, R. V., Sartori, R., and Franco, M. M., Characterization of allele-specific expression of the X-linked gene MAO-A in trophectoderm cells of bovine embryos produced by somatic cell nuclear transfer, vol. 14, pp. 12128-12136, 2015.

Somatic cell nuclear transfer (SCNT) may affect epigenetic mechanisms and alter the expression of genes related to embryo development and X chromosome inactivation (XCI). We characterized allele-specific expression of the X-linked gene monoamine oxidase type A (MAO-A) in the trophectoderm (TF) of embryos produced by SCNT. Total RNA was isolated from individual biopsies (N = 25), and the allele-specific expression assessed by reverse transcription-polymerase chain reaction-restriction fragment length polymorphism.

Molecular cloning, characterization and expression analysis of WAG-1 in the pistillody line of common wheat

Q. H. Wang, Yang, Z. J., Wei, S. H., Jiang, Z. Y., Yang, Y. F., Hu, Z. S., Sun, Q. X., and Peng, Z. S., Molecular cloning, characterization and expression analysis of WAG-1 in the pistillody line of common wheat, vol. 14, pp. 12455-12465, 2015.

Wheat WAG-1 is a C-class MADS-box gene, which is orthologous to AGAMOUS in Arabidopsis. In this study, we report the cloning, characterization, and expression patterns of WAG-1 in the pistillody mutant HTS-1 and its sib-line CSTP. The cDNA of WAG-1 was found to be 765 bp in length, which was equal to the length of its open reading frame, encoding 254 amino acids. The location of WAG-1 revealed that it has three homologous genes from the short arm of chromosome 1A, 1B, and 1D.

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