Cloning

Molecular cloning, tissue expression pattern, and copy number variation of porcine SCUBE3

X. Liu, Wang, L. G., Zhang, L. C., Yan, H., Zhao, K. B., Liang, J., Li, N., Pu, L., Zhang, T., Wang, L. X., Liu, X., Wang, L. G., Zhang, L. C., Yan, H., Zhao, K. B., Liang, J., Li, N., Pu, L., Zhang, T., and Wang, L. X., “Molecular cloning, tissue expression pattern, and copy number variation of porcine SCUBE3”, vol. 15, p. -, 2016.

The signal peptide CUB EGF-like domain-containing protein 3 (SCUBE3) gene is a member of SCUBE gene family and plays important roles in bone cell biology and the determination of limb bone length. In this study, the full-length transcript of porcine SCUBE3 was cloned using reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends. The full-length sequence of porcine SCUBE3 cDNA was 4131 base pairs and included 21 exons.

Molecular cloning and expression analysis of MAT1 gene in black tiger shrimp (Penaeus monodon)

Y. Wang, Fu, M. J., Zhao, C., Bao, W. Y., Zhou, F. L., Yang, Q. B., Jiang, S. G., Qiu, L. H., Wang, Y., Fu, M. J., Zhao, C., Bao, W. Y., Zhou, F. L., Yang, Q. B., Jiang, S. G., and Qiu, L. H., “Molecular cloning and expression analysis of MAT1 gene in black tiger shrimp (Penaeus monodon)”, vol. 15, p. -, 2016.

MAT1 (ménage à trois 1), an assembly factor and targeting subunit of the CDK-dependent kinase (CAK), can regulate the cell cycle, transcription, and DNA repair. This study was intended to investigate the role of MAT1 in the reproductive maturation of black tiger shrimp (Penaeus monodon). In this study, the P. monodon MAT1 (PmMAT1) gene was identified and characterized. The full-length cDNA of PmMAT1 was 1490 bp in length with an open-reading frame of 993 bp corresponding to 330 amino acids.

Molecular characterization and tissue expression profile of porcine Ephrin-B2

D. Xu, Ma, H. M., Xu, D., and Ma, H. M., “Molecular characterization and tissue expression profile of porcine Ephrin-B2”, vol. 15, p. -, 2016.

Ephrin-B2 (EFNB2) is a signaling molecule that plays an important role in cell adhesion, proliferation, and migration in humans. However, little is known about this molecule in pigs. In order to investigate whether EFNB2 is associated with the skeletal muscle in pigs, we cloned the full-length cDNA of EFNB2 (GenBank accession No. KF500033) from the longissimus dorsi muscle of Yorkshire pigs by rapid amplification of cDNA ends.

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