The goal of this study was to identify the differences in gene expression between adenocarcinoma samples and colonic normal mucosa of a Chinese population. Gene expression libraries of adenocarcinoma and normal colonic mucosa were generated by serial analysis of gene expression (SAGE). Expression tags were obtained from sequencing results using the SAGE2000 software. Representative genes of tags were determined based on the SAGEmap from NCBI. Expressed genes were selected and their expression in different libraries was compared using the SPSS 17.0 software.