Comet assay

Evaluation of pH effects on genomic integrity in adipose-derived mesenchymal stem cells using the comet assay

L. C. Hermeto, Oliveira, R. J., Matuo, R., Jardim, P. H. A., DeRossi, R., Antoniolli, A. C. M. B., Deffune, E., Evaristo, T. C., and Santana, Á. E., Evaluation of pH effects on genomic integrity in adipose-derived mesenchymal stem cells using the comet assay, vol. 14, pp. 339-348, 2015.

The use of mesenchymal stem cells (MSCs) in experimental, clinical, and therapeutic trials has grown in recent years. However, the issue remains of whether these procedures are completely safe for transplant patients. Therefore, this study was designed and carried out with the aim of evaluating two different comet assay protocols for genomic damage pattern analysis in MSCs derived from adipose tissue. The analyzed and interpreted results suggest that genetic testing is needed to support clonal expansion safety in cell therapy procedures with MSCs.

Assessment of the cytotoxic, genotoxic, and mutagenic potential of Acrocomia aculeata in rats

G. K. Traesel, Castro, L. H. A., Silva, P. V. B., Muzzi, R. M., Kassuya, C. A. L., Arena, A. C., and Oesterreich, S. A., Assessment of the cytotoxic, genotoxic, and mutagenic potential of Acrocomia aculeata in rats, vol. 14, pp. 585-596, 2015.

Acrocomia aculeata (Jacq.) Lodd. ex Mart. is a plant species commonly used as a foodstuff and also for treating diseases, since it contains high concentrations of antioxidant compounds and monounsaturated fatty acids. Considering its ethnopharmacological relevance, the aim of the present study was to assess the cytotoxic, genotoxic, and mutagenic effects of an oil extracted from the pulp of A. aculeata (OPAC) in rats. In addition, a chromatographic characterization of the fatty acids present in OPAC was performed.

6-Dimethylaminopurine and cyclohexamide are mutagenic and alter reproductive performance and intrauterine development in vivo

R. J. Oliveira, Mantovani, M. S., Pesarini, J. R., Mauro, M. O., da Silva, A. F., Souza, T. R., and Ribeiro, L. R., 6-Dimethylaminopurine and cyclohexamide are mutagenic and alter reproductive performance and intrauterine development in vivo, vol. 14, pp. 834-849, 2015.

The compounds 6-dimethylaminopurine (6-DMAP) and cyclohexamide (CHX) are currently used to stimulate the development of embryos produced by nuclear transfer in the production of cloned mammals with a great deal success. This study investigated the effects of 6-DMAP and CHX in vivo using biological assays to evaluate reproductive performance in females, teratogenesis, and mutagenesis.

Antigenotoxic and antimutagenic effects of Schinus terebinthifolius Raddi in Allium cepa and Swiss mice: A comparative study

L. E. S. Fedel-Miyasato, Formagio, A. S. N., Auharek, S. A., Kassuya, C. A. L., Navarro, S. D., Cunha-Laura, A. L., Monreal, A. C. D., Vieira, M. C., and Oliveira, R. J., Antigenotoxic and antimutagenic effects of Schinus terebinthifolius Raddi in Allium cepa and Swiss mice: A comparative study, vol. 13, pp. 3411-3425, 2014.

It is estimated that 60% of anticancer drugs are derived directly or indirectly from medicinal plants. Schinus terebinthifolius Raddi (Anacardiaceae) is traditionally used in Brazilian medicine to treat inflammation, ulcers, and tumors. Because of the need to identify new antimutagenic agents and to determine their mechanism of action, this study evaluated the chemopreventive activity of the methanolic extract from leaves of S.

Mutagenic potential of Cordia ecalyculata alone and in association with Spirulina maxima for their evaluation as candidate anti-obesity drugs

R. P. Araldi, Rechiutti, B. M., Mendes, T. B., Ito, E. T., and Souza, E. B., Mutagenic potential of Cordia ecalyculata alone and in association with Spirulina maxima for their evaluation as candidate anti-obesity drugs, vol. 13, pp. 5207-5220, 2014.

Obesity is one of the most important nutritional disorders, and can be currently considered as an epidemic. Although there are few weight reduction drugs available on the market, some new drug candidates have been proposed, including Cordia ecalyculata, a Brazilian plant with anorectic properties, and Spirulina maxima, a cyanobacterium with antioxidant and anti-genotoxic activity. In this study, we evaluated the mutagenic potential of C. ecalyculata at doses of 150, 300, and 500 mg/kg alone and in association with S.

Assessing the genotoxicities of sparteine and compounds isolated from Lupinus mexicanus and L. montanus seeds by using comet assay

M. R. Silva, Alvarez, C. M., García, P. M., and Ruiz, M. A., Assessing the genotoxicities of sparteine and compounds isolated from Lupinus mexicanus and L. montanus seeds by using comet assay, vol. 13, pp. 10510-10517, 2014.

The genus Lupinus is widely distributed. Its seeds are used for animal and human food, and Lupinus possesses pharmacological potential because of its high content of quinolizidine alkaloids and flavonoids; however, there is little available information about its genotoxicity. We used the comet assay and staminal nuclei of Tradescantia (clone 4430) to evaluate the in vitro genotoxicity of 4 concentrations (0.01, 0.1, 0.5, and 1.0 mM) of alkaloid extracts of Lupinus mexicanus and Lupinus montanus, flavonoids of L.

Association of GSTM1 and GSTT1 with ageing in auto repair shop workers

S. A. Eshkoor, Marashi, S. J., Ismail, P., Rahman, S. A., Mirinargesi, M., Adon, M. Y., and Devan, R. V., Association of GSTM1 and GSTT1 with ageing in auto repair shop workers, vol. 11, pp. 1486-1496, 2012.

We evaluated the possible influence of glutathione S-transferase mu (GSTM1) and glutathione S-transferase theta (GSTT1) genes on genetic damage due to occupational exposure, which contributes to accelerate ageing. This study was conducted on 120 car auto repair workshop workers exposed to occupational hazards and 120 controls without this kind of exposure. The null and non-null genotypes of GSTM1 and GSTT1 genes were determined by multiplex PCR.

Genotoxicity of the medicinal plant Maytenus robusta in mammalian cells in vivo

T. M. Raymundo, Favilla, M., Niero, R., Andrade, S. F., and Maistro, E. L., Genotoxicity of the medicinal plant Maytenus robusta in mammalian cells in vivo, vol. 11, pp. 2847-2854, 2012.

Plants belonging to the Celastraceae family have been used in traditional medicine for their analgesic, anti-inflammatory and anti-ulcerogenic properties, among others. Maytenus ilicifolia is the principal species of this family, and is used in the treatment of gastric ulcers. However, owing to its inadequate management in Brazil, the species is becoming extinct and is being substituted with Maytenus robusta, which also displays gastroprotective activity. The aim of this study was to evaluate the genotoxic effects of M.

Damaging effects of water-borne cadmium chloride on DNA of lung cells of immature mice

X. F. Yang, Ge, Y. M., Zhang, H. T., Ning, H. M., Jiang, J. Q., Qi, Y. H., and Wang, Z. L., Damaging effects of water-borne cadmium chloride on DNA of lung cells of immature mice, vol. 11, pp. 4323-4329, 2012.

We investigated the effects of cadmium on lung cell DNA in immature mice. The mice were randomly divided into four groups: control group, low-dose group (1/100 LD50), middle-dose group (1/50 LD50), and high-dose group (1/25 LD50); they were supplied with cadmium chloride or control water for 40 days. Lung cells collected from sacrificed mice were used to evaluate the extent of DNA damage by comet assay.

Genotoxic evaluation of the antimalarial drugs artemisinin and artesunate in human HepG2 cells and effects on CASP3 and SOD1 gene expressions

I. Aquino, Tsuboy, M. S. F., Marcarini, J. C., Mantovani, M. S., Perazzo, F. F., and Maistro, E. L., Genotoxic evaluation of the antimalarial drugs artemisinin and artesunate in human HepG2 cells and effects on CASP3 and SOD1 gene expressions, vol. 12, pp. 2517-2527, 2013.

The malaria treatment recommended by the World Health Organization involves medicines derived from artemisinin, an active compound extracted from the plant Artemisia annua, and some of its derivatives, such as artesunate. Considering the lack of data regarding the genotoxic effects of these compounds in human cells, the objective of this study was to evaluate the cytotoxicity and genotoxicity, and expressions of the CASP3 and SOD1 genes in a cultured human hepatocellular liver carcinoma cell line (HepG2 cells) treated with artemisinin and artesunate.

Pages

Subscribe to Comet assay