Conservation

Mendelian inheritance, genetic linkage, and genotypic disequilibrium at microsatellite loci in Genipa americana L. (Rubiaceae)

R. O. Manoel, Freitas, M. L. M., Tambarussi, E. V., Cambuim, J., Moraes, M. L. T., and Sebbenn, A. M., “Mendelian inheritance, genetic linkage, and genotypic disequilibrium at microsatellite loci in Genipa americana L. (Rubiaceae)”, vol. 14, pp. 8161-8169, 2015.

Genipa americana is a tropical tree species that is widely distributed in the humid tropical and subtropical regions of Central and South America. This study investigated Mendelian inheritance, ge­netic linkage, and genotypic disequilibrium at six microsatellite loci developed for G. americana. Adult trees (188) and regenerants (163) were sampled and genotyped in a fragmented population of the spe­cies. We also genotyped open-pollinated seeds from 12 seed-trees dur­ing reproductive events in 2010 and 2011.

Isolation and characterization of microsatellite markers of sea cucumber Stichopus horrens

Z. B. Li, Dai, G., Shangguan, J. B., Ning, Y. F., Li, Y. Y., Chen, R. B., Yuan, Y., and Huang, Y. S., “Isolation and characterization of microsatellite markers of sea cucumber Stichopus horrens”, vol. 14. pp. 8496-8499, 2015.

Curry fish (Stichopus horrens) is a tropical holothurian species and is widely distributed in the India-West Pacific. In the present study, 9 polymorphic microsatellite loci were isolated and characterized for S. horrens. These loci were tested in 30 individuals from Hainan Island in China. The number of alleles ranged from 2 to 5. The polymorphism information content ranged from 0.348-0.584. The levels of observed and expected heterozygosities varied from 0.1500-0.8000 and from 0.2014-0.5000, respectively.

Genetic diversity of wild Prunus cerasifera Ehrhart (wild cherry plum) in China revealed by simple-sequence repeat markers

Y. Zhao, Li, Y., Liu, Y., and Yang, Y. F., “Genetic diversity of wild Prunus cerasifera Ehrhart (wild cherry plum) in China revealed by simple-sequence repeat markers”, vol. 14, pp. 8407-8413, 2015.

Simple-sequence repeat (SSR) markers were employed to assess the genetic diversity of wild Prunus cerasifera Ehrhart (wild cherry plum) in China. Fourteen SSR primer pairs generated a total of 94 alleles (90 were polymorphic, accounting for 95.74%), with a mean of 6.71 alleles per locus. The number of alleles detected at each locus ranged from 2 at BPPCT 028 to 13 at BPPCT 002, with an average of 6.71 alleles per locus.

Use of microsatellite markers to assign goats to their breeds

R. S. Aljumaah, Alobre, M. M., and Al-Atiyat, R. M., “Use of microsatellite markers to assign goats to their breeds”, vol. 14, pp. 9071-9080, 2015.

We investigated the potential of 17 microsatellite markers for assigning Saudi goat individuals to their breeds. Three local breeds, Bishi, Jabali, and Tohami were genotyped using these markers, and Somali goats were used as a reference breed. The majority of alleles were shared between the breeds, except for some that were specific to each breed. The Garza-Williamson index was lowest in the Bishi breed, indicating that a recent bottleneck event occurred.

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