cpDNA

Genetic diversity and differentiation in Prunus species (Rosaceae) using chloroplast and mitochondrial DNA CAPS markers

S. Ben Mustapha, Ben Tamarzizt, H., Baraket, G., Abdallah, D., and A. Hannachi, S., Genetic diversity and differentiation in Prunus species (Rosaceae) using chloroplast and mitochondrial DNA CAPS markers, vol. 14, pp. 4177-4188, 2015.

Chloroplast (cpDNA) and mitochondrial DNA (mtDNA) were analyzed to establish genetic relationships among Tunisian plum cultivars using the polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) technique. Two mtDNA regions (nad 1 b/c and nad 4 1/2) and a cpDNA region (trnL-trnF) were amplified and digested using restriction enzymes. Seventy and six polymorphic sites were revealed in cpDNA and mtDNA, respectively. As a consequence, cpDNA appears to be more polymorphic than mtDNA.

Usefulness of cpDNA markers for phylogenetic and phylogeographic analyses of closely related cactus species

I. A. S. Bonatelli, Zappi, D. C., Taylor, N. P., and Moraes, E. M., Usefulness of cpDNA markers for phylogenetic and phylogeographic analyses of closely related cactus species, vol. 12, pp. 4579-4585, 2013.

Although plastid DNA has been widely explored as a marker of choice for phylogeny and phylogeography studies, little is known about its utility for examining relationships between closely related species. The slow evolutionary rates inherent to chloroplast (cp) DNA make it difficult to perform lower level taxonomic analyses, particularly at the population level.

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