Cre recombinase

Decreased expression of humanized Fat-1 in porcine fetal fibroblasts following deletion of PGK-neomycin resistance

X. J. Han, Liang, H., Yun, T., Zhao, Y. H., Zhang, M. L., Zhao, L. H., Li, R. F., and Li, X. L., Decreased expression of humanized Fat-1 in porcine fetal fibroblasts following deletion of PGK-neomycin resistance, vol. 14, pp. 11594-11604, 2015.

The neomycin-resistance (neor) gene is widely used as a selectable marker in eukaryotic expression vectors; however, its expression often affects that of target genes. Cre recombinase recognizes LoxP sites, leading to site-specific recombination and deletion of DNA and RNA between two LoxP sites. In the present study, a humanized Fat-1 gene (hFat-1) was generated by DNA Works and used to construct a pC-PGK-neor-hfat-1 expression vector, in which PGK-neor was flanked by two LoxP sites.

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