Cross-species amplification

Development of polymorphic SSR markers in the razor clam (Sinonovacula constricta) and cross-species amplification

Y. H. Dong, Yao, H. H., Sun, C. S., Lv, D. M., Li, M. Q., Lin, Z. H., Dong, Y. H., Yao, H. H., Sun, C. S., Lv, D. M., Li, M. Q., Lin, Z. H., Dong, Y. H., Yao, H. H., Sun, C. S., Lv, D. M., Li, M. Q., and Lin, Z. H., “Development of polymorphic SSR markers in the razor clam (Sinonovacula constricta) and cross-species amplification”, vol. 15, p. -, 2016.

Next-generation sequencing provides large-scale sequencing data with relative ease and at a reasonable cost, making it possible to identify a large amount of SSR markers in a timely and cost-effective manner. On the basis of the transcriptome database of Sinonovacula constricta obtained by Illumina/Solexa pyrosequencing, 60 polymorphic SSR markers were developed and characterized in 30 individuals. The number of alleles per polymorphic locus ranged from 2 to 7 with an average of 3.75 alleles.

Development of novel DNA markers for genetic analysis of grey hamsters by cross-species amplification of microsatellites

C. Wang, Zhang, S. J., Du, X. Y., Xu, Y. M., Huo, X. Y., Liao, L. F., and Chen, Z. W., “Development of novel DNA markers for genetic analysis of grey hamsters by cross-species amplification of microsatellites”, vol. 14, pp. 14339-14347, 2015.

The grey hamster has been used in biomedical research for decades. However, effective molecular methods for evaluating the genetic structure of this species are lacking, which hinders its wider usage. In this study, we employed cross-amplification of microsatellite loci of species within the same genus by polymerase chain reaction. Loci screened included 107 from the Mongolian gerbil (MG) and 60 from the Chinese hamster (CH); of these, 15 polymorphic loci were identified for the grey hamster.

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