Cultivar identification diagram

Identification of apple cultivars on the basis of simple sequence repeat markers

G. S. Liu, Zhang, Y. G., Tao, R., Fang, J. G., and Dai, H. Y., Identification of apple cultivars on the basis of simple sequence repeat markers, vol. 13, pp. 7377-7387, 2014.

DNA markers are useful tools that play an important role in plant cultivar identification. They are usually based on polymerase chain reaction (PCR) and include simple sequence repeats (SSRs), inter-simple sequence repeats, and random amplified polymorphic DNA. However, DNA markers were not used effectively in the complete identification of plant cultivars because of the lack of known DNA fingerprints. Recently, a novel approach called the cultivar identification diagram (CID) strategy was developed to facilitate the use of DNA markers for separate plant individuals.

Identification of grapevine rootstock cultivars using expressed sequence tag-simple sequence repeats

X. C. Fan, Chu, J. Q., Liu, C. H., Sun, X., and Fang, J. G., Identification of grapevine rootstock cultivars using expressed sequence tag-simple sequence repeats, vol. 13, pp. 7649-7657, 2014.

Grapevine (Vitis) rootstock varieties or cultivars are used to confer resistance and tolerance to insect and disease pests, unfavorable soil conditions, and other environmental conditions to cultivars that are susceptible to these conditions but otherwise have desired properties. The need to genotype and thoroughly identify grapevine rootstock varieties in the grape industry has become increasingly critical as more and more varieties are bred or selected.

A novel strategy for identification of 47 pomegranate (Punica granatum) cultivars using RAPD markers

Y. P. Zhang, Tan, H. H., Cao, S. Y., Wang, X. C., Yang, G., and Fang, J. G., A novel strategy for identification of 47 pomegranate (Punica granatum) cultivars using RAPD markers, vol. 11, pp. 3032-3041, 2012.

DNA marker can be used for precise plant cultivar identification. However, DNA markers have often not been used effectively for the identification of plant cultivars due to a lack of an effective analysis strategy. We used a novel strategy for effective identification of plant individuals based on a new way of recording DNA fingerprints of the genotyped plants; a cultivar identification diagram can be manually generated and used as key reference information for quick identification of plant and/or seed samples.

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