Cut-and-paste-based cloning strategy

Cut-and-paste-based cloning strategy for large gene site-directed mutagenesis

C. Wang, Wang, T. Y., Zhang, L. Y., Gao, X. J., Wang, X. W., and Jin, C. J., Cut-and-paste-based cloning strategy for large gene site-directed mutagenesis, vol. 14, pp. 5585-5591, 2015.

Site-directed mutagenesis is an essential technique for investigating the mechanisms of gene regulation on a molecular level, as well as for exploring post-translational modifications and functional structure at the protein level. Polymerase chain reaction combining in vitro synthesis of oligonucleotide primers allows for site-directed mutation to be performed with ease. However, site-directed mutagenesis is difficult when larger plasmids are involved.

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