Influence of sirolimus-induced TGF-β secretion on mouse Treg cell proliferation
“Influence of sirolimus-induced TGF-β secretion on mouse Treg cell proliferation”, vol. 14, pp. 18569-18579, 2015.
, We examined the effects of co-culturing CD4+ CD25+ Treg cells with sirolimus or cyclosporin A on Treg cell proliferation and differentiation and on transforming growth factor-β (TGF-β) and Foxp3 expression. CD4+ CD25+ Treg cells were harvested from mononuclear cells of spleens of C57BL/6 mice using immunomagnetic beads and divided into control, sirolimus, and cyclosporine groups. Following a 96-h co-culture, Treg cells were assayed by flow cytometry.