Cyclosporin A

Influence of sirolimus-induced TGF-β secretion on mouse Treg cell proliferation

J. N. Li, Li, J. X., Huang, H. L., Zhu, X. L., Ding, H., Huang, C. F., Lin, J., Huang, J. G., Wu, Z. C., Ashraf, M., Wang, Y. G., Li, X. K., Zheng, S. Y., Chen, J. M., Guo, H. M., Zhuang, J., and Zhu, P., Influence of sirolimus-induced TGF-β secretion on mouse Treg cell proliferation, vol. 14, pp. 18569-18579, 2015.

We examined the effects of co-culturing CD4+ CD25+ Treg cells with sirolimus or cyclosporin A on Treg cell proliferation and differentiation and on transforming growth factor-β (TGF-β) and Foxp3 expression. CD4+ CD25+ Treg cells were harvested from mononuclear cells of spleens of C57BL/6 mice using immunomagnetic beads and divided into control, sirolimus, and cyclosporine groups. Following a 96-h co-culture, Treg cells were assayed by flow cytometry.

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