CYP

Involvement of CYP1A1, GST, 72TP53 polymorphisms in the pathogenesis of thyroid nodules

A. A. S. Reis, Silva, D. M., Curado, M. P., and da Cruz, A. D., Involvement of CYP1A1, GST, 72TP53 polymorphisms in the pathogenesis of thyroid nodules, vol. 9, pp. 2222-2229, 2010.

Specific genotypes appear to be related to the development of thyroid disease. We examined whether polymorphisms of the genes CYP1A1, GSTM1, GSTT1, and TP53 at codon 72 are associated with increased risk for thyroid nodules. Blood samples were obtained from 122 thyroid patients with nodules and from 134 healthy control individuals from Goiânia city, GO, Brazil. We found no significant association of CYP1A1m1 and CYP1A1m2 genotypes with thyroid diseases (P > 0.05).

N-nitrosodiethylamine genotoxicity evaluation: a cytochrome P450 induction study in rat hepatocytes

C. A. F. Aiub, Gadermaier, G., Silva, I. O., Felzenszwalb, I., Pinto, L. F. R., Ferreira, F., and Eckl, P., N-nitrosodiethylamine genotoxicity evaluation: a cytochrome P450 induction study in rat hepatocytes, vol. 10, pp. 2340-2348, 2011.

In rats, N-nitrosodiethylamine (NDEA) induces tumors mainly in the liver. This could be because various enzymes are responsible for the metabolic activation of NDEA, besides the hepatic NDEA metabolizing enzyme, CYP2E1. We examined NDEA genotoxicity and cytotoxicity in primary cultures of female rat hepatocytes; we also looked at how it affected CYP mRNA expression. Single incubation with 0.9% NaCl resulted in a mean of 0.2% apoptotic cells, which doubled with 105 μg NDEA/mL. The frequency of necrosis with NDEA treatment was also doubled.

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