Cytochrome b

Prospective molecular markers for the identification of illegally traded angelsharks (Squatina) and dolphin (Sotalia guianensis)

L. H. O. Falcão, Furtado-Neto, M. A. A., Maggioni, R., and Faria, V. V., Prospective molecular markers for the identification of illegally traded angelsharks (Squatina) and dolphin (Sotalia guianensis), vol. 13, pp. 9710-9717, 2014.

Endangered angelsharks and a protected dolphin species are illegally traded in Brazil. In this study, we determined prospective molecular markers for detecting these species in the trade of angelshark carcasses and ‘dolphin’ eyeball amulets. We compiled publicly available as well as new and unpublished cytochrome b (cyt b) DNA sequences for species involved in these trades. These sequences were digested in silico using restriction enzymes.

Amplifiability of mitochondrial, microsatellite and amelogenin DNA loci from fecal samples of red brocket deer Mazama americana (Cetartiodactyla, Cervidae)

M. L. Oliveira and Duarte, J. M. B., Amplifiability of mitochondrial, microsatellite and amelogenin DNA loci from fecal samples of red brocket deer Mazama americana (Cetartiodactyla, Cervidae), vol. 12, pp. 44-52, 2013.

We tried to amplify mitochondrial, microsatellite and amelogenin loci in DNA from fecal samples of a wild Mazama americana population. Fifty-two deer fecal samples were collected from a 600-ha seasonal semideciduous forest fragment in a subtropical region of Brazil (21°20'S, 47°17'W), with the help of a detection dog; then, stored in ethanol and georeferenced. Among these samples 16 were classified as “fresh” and 36 as “non-fresh”. DNA was extracted using the QIAamp® DNA Stool Mini Kit. Mitochondrial loci were amplified in 49 of the 52 samples.

 Molecular evolution and adaptation of the mitochondrial cytochrome b gene in the subgenus Martes

B. Li, Malyarchuk, B., He, X. B., and Derenko, M.,  Molecular evolution and adaptation of the mitochondrial cytochrome b gene in the subgenus Martes, vol. 12, pp. 3944-3954, 2013.

Martes species represent a typical example of rapid evolutionary radiation and a recent speciation event. To identify regions of the genome that experienced adaptive evolution, which might provide clues to their functional importance and may be informative about the features that make each species unique, we sought evidence of molecular adaptation in the mitochondrial DNA (mtDNA) cytochrome b gene in the subgenus Martes.

 DNA-based and geometric morphometric analysis to validate species designation: a case study of the subterranean rodent Ctenomys bicolor

J. F. B. Stolz, Gonçalves, G. L., Leipnitz, L., and Freitas, T. R. O.,  DNA-based and geometric morphometric analysis to validate species designation: a case study of the subterranean rodent Ctenomys bicolor, vol. 12, pp. 5023-5037, 2013.

The genus Ctenomys (Rodentia: Ctenomyidae) shows several taxonomic inconsistencies. In this study, we used an integrative approach including DNA sequences, karyotypes, and geometric morphometrics to evaluate the taxonomic validity of a nominal species, Ctenomys bicolor, which was described based on only one specimen in 1912 by Miranda Ribeiro, and since then neglected. We sampled near the type locality assigned to this species and collected 10 specimens.

Phylogenetic relationships of the Orang Asli and Iban of Malaysia based on maternal markers

K. C. Ang, Leow, J. W. H., Yeap, W. K., Hood, S., Mahani, M. C., and Md-Zain, B. M., Phylogenetic relationships of the Orang Asli and Iban of Malaysia based on maternal markers, vol. 10, pp. 640-649, 2011.

Malaysia remains as a crossroad of different cultures and peoples, and it has long been recognized that studying its population history can provide crucial insight into the prehistory of Southeast Asia as a whole. The earliest inhabitants were the Orang Asli in Peninsular Malaysia and the indigenous groups in Sabah and Sarawak. Although they were the earliest migrants in this region, these tribes are divided geographically by the South China Sea.

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