Cytogenetic

Chromosome heteromorphisms are more frequent in couples with recurrent abortions

H. Akbas, İsi, H., İsi, H., Oral, D., Turkyılmaz, A., Kalkanlı-Taş, S., Simsek, S., Balkan, M., Sakar, M. N., Fidanboy, M., Alp, M. N., and Budak, T., Chromosome heteromorphisms are more frequent in couples with recurrent abortions, vol. 11, pp. 3847-3851, 2012.

Chromosomal heteromorphism is considered a variant of a normal karyotype, but it is more frequent in couples with repeated miscarriages. We investigated chromosomal heteromorphism in couples with repeated miscarriages in comparison with a control group. A total of 455 couples who applied to our genetic diagnosis laboratory in Diyarbakir, Turkey, were evaluated for chromosome heteromorphisms; 221 of these couples (the study group) had recurrent abortions and 234 of them (the control group) had no history of abortions and had at least one living child.

Prognostic implication of molecular aberrations in cytogenetically normal acute myeloid leukemia patients receiving allogeneic hematopoietic stem cell transplantation

Y. C. Liu, Hsiao, H. H., Lin, P. M., Yang, W. C., Chang, C. S., Liu, T. C., Hsu, J. F., Yang, M. Y., and Lin, S. F., Prognostic implication of molecular aberrations in cytogenetically normal acute myeloid leukemia patients receiving allogeneic hematopoietic stem cell transplantation, vol. 12, pp. 5414-5423, 2013.

Different molecular aberrations can be discriminated into certain prognostic subgroups in cytogenetically normal acute myeloid leukemia (CN-AML) patients but their impact on allogeneic hematopoietic stem cell transplantation (allo-HSCT) remains controversial and studies from Asian populations are lacking. Forty-two adult non-M3 AML patients receiving allo-HSCT from 2002 to 2009 in southern Taiwan were retrospectively reviewed for survey, 23 (54.7%) of whom were CN-AML. NPM1, FLT3-ITD, and CEBPA were analyzed.

Cytogenetic analysis of 4216 patients referred for suspected chromosomal abnormalities in Southeast Turkey

M. Balkan, Akbas, H., İsi, H., İsi, H., Oral, D., Turkyılmaz, A., Kalkanli, S., Simsek, S., Fidanboy, M., Alp, M. N., Gedik, A., and Budak, T., Cytogenetic analysis of 4216 patients referred for suspected chromosomal abnormalities in Southeast Turkey, vol. 9, pp. 1094-1103, 2010.

We reviewed cytogenetic studies performed on 4216 patients who were referred to the Cytogenetics Unit at Dicle University Hospital, Diyarbair, Southeast Turkey, between 2000 and 2009. The cases were grouped according to the reason of referral for cytogenetic analysis. The frequencies of the different types of numerical and structural abnormalities were determined, and the relative frequency of cases with abnormal karyotypes was calculated in each group. The most common reason for requesting cytogenetic testing was referral for Down syndrome and for repeated abortions.

Improvements in cytological preparations for fluorescent in situ hybridization in Passiflora

M. M. Souza, Urdampilleta, J. D., and Forni-Martins, E. R., Improvements in cytological preparations for fluorescent in situ hybridization in Passiflora, vol. 9, pp. 2148-2155, 2010.

Cytological preparations for the fluorescent in situ hybridization (FISH) technique require cytoplasm-free metaphases, with well-spread chromosomes, for the localization of DNA sequences and chromosome mapping. We tested various procedures for FISH analysis of Passiflora cacaoensis, P. gardneri and hybrid F1 progeny of P. gardneri x P. gibertii. Two treatments with four enzymes and three incubation times were compared. The material was treated with 1.0 M HCl before enzymatic digestion.

Chromosomal characteristics and karyotype evolution of Oxyopidae spiders (Araneae, Entelegynae)

L. M. Stávale, Schneider, M. C., Brescovit, A. D., and Cella, D. M., Chromosomal characteristics and karyotype evolution of Oxyopidae spiders (Araneae, Entelegynae), vol. 10, pp. 752-763, 2011.

We made a cytogenetic analysis of four species of Oxyopidae and compared it with the karyotype data of all species of this family. In Hamataliwa sp, the mitotic cells showed 2n♂ = 26+X1X2 and telocentric chromosomes. The 2n♂ = 28, which has been described for only one oxyopid spider, is the highest diploid number reported for this family. Peucetia species exhibited distinct karyotype characteristics, i.e., 2n♂ = 20+X1X2 in P. flava and 2n♂ = 20+X in P. rubrolineata, revealing interspecific chromosome variability within this genus.

Simple method for culture of peripheral blood lymphocytes of Testudinidae

T. L. Silva, Silva, M. I. A., Venancio, L. P. R., Zago, C. E. S., Moscheta, V. A. G., Lima, A. V. B., Vizotto, L. D., Santos, J. R., Bonini-Domingos, C. R., and Azeredo-Oliveira, M. T. V., Simple method for culture of peripheral blood lymphocytes of Testudinidae, vol. 10, pp. 3020-3025, 2011.

We developed and optimized a simple, efficient and inexpensive method for in vitro culture of peripheral blood lymphocytes from the Brazilian tortoise Chelonoidis carbonaria (Testudinidae), testing various parameters, including culture medium, mitogen concentration, mitotic index, culture volume, incubation time, and mitotic arrest. Peripheral blood samples were obtained from the costal vein of four couples.

Cytogenetic and random ampliied polymorphic DNA analysis of Leptodactylus species from rural and urban environments (Anura, Amphibia)

M. P. Arruda and Morielle-Versute, E., Cytogenetic and random ampliied polymorphic DNA analysis of Leptodactylus species from rural and urban environments (Anura, Amphibia), vol. 7, pp. 161-176, 2008.

Cytogenetic and random amplified polymorphic DNA analyses carried out in the species Leptodactylus podicipinus, L. ocellatus, L. labyrinthicus, and L. fuscus from rural and urban habitats of the northwest region of São Paulo State, Brazil, showed that the karyotypes (2n = 22), constitutive heterochromatin distribution and nucleolus organizer region (NOR) location did not differ between the populations from the two environments.

DNA extraction from fixed cytogenetic cell suspensions

M. R. Amorim, Vargas, F. R., Junior, J. C. Llerena, and Pombo-de-Oliveira, M. S., DNA extraction from fixed cytogenetic cell suspensions, vol. 6, pp. 500-503, 2007.

We developed a procedure for DNA extraction from small volumes of fixed cell suspensions previously prepared for conventional cytogenetic analysis. Good quality DNA was isolated with a fast and simple protocol using DNAzol reagent. This provided suitable DNA for various types of molecular analyses, including polymerase chain reaction, restriction fragment length polymorphism, denaturing high-performance liquid chromatography, and direct sequencing.

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