Cytogenetics

Molecular and chromosomal mutations among children with B-lineage lymphoblastic leukemia in Brazil’s Federal District

D. R. Mesquita, Córdoba, J. C., Magalhães, Í. Q., Córdoba, M. S., Oliveira, J. R. C., Gonçalves, A., Ferrari, I., and Martins-de-Sá, C., Molecular and chromosomal mutations among children with B-lineage lymphoblastic leukemia in Brazil’s Federal District, vol. 8, pp. 345-353, 2009.

Acute lymphoblastic leukemia (ALL) accounts for approximately 80% of all acute leukemias during childhood. Chromosomal anomalies resulting from gene fusion, which are frequent in leukemias, create hybrid transcripts, the great majority of which encode transcription factors. We analyzed 88 pediatric patients (median age 7.3 years) who had B-lineage acute lymphoblastic leukemia (B-ALL), using reverse transcriptase-polymerase chain reaction, to look for gene fusion transcripts of TEL/AML1, E2A/PBX1, BCR/ABL p190, and MLL/AF4.

Cytogenetic findings in an epithelioid sarcoma with angiomatoid features.A case report

M. S. Brassesco, Valera, E. T., Castro-Gamero, A. M., Moreno, D. A., Silveira, T. P., Mori, B. M., Engel, E. E., Scrideli, C. A., and Tone, L. G., Cytogenetic findings in an epithelioid sarcoma with angiomatoid features.A case report, vol. 8. pp. 1211-1217, 2009.

Epithelioid sarcoma is a rare, aggressive soft tissue tumor of unknown histogenesis showing predominantly epithelioid cytomorphology. We conducted a conventional and molecular cytogenetic study of a 27-year-old male with epithelioid sarcoma with angiomatoid features. Cytogenetic analysis of epithelioid sarcoma metaphase spreads by GTG-banding revealed a diploid chromosome complement with structural and numerical aberrations.

Cytogenetic characteristics of patients with signs and symptoms of myelodysplastic syndromes in the State of Pará, Brazil

G. Rebouças Pinto, Overal, D. James, Van Den Berg, A. Virgínia, Lemos, J. Alexandre, Smith, Mde Arruda, Smith, Mde Arruda, Burbano, R. Rodríguez, and de Moraes, L. Silva, Cytogenetic characteristics of patients with signs and symptoms of myelodysplastic syndromes in the State of Pará, Brazil, vol. 4, pp. 18-30, 2005.

The myelodysplastic syndromes (MDS) are clonal hematopoietic diseases characterized by medullary dysplasia, cytopenias, and frequent evolution to acute myeloid leukemia. In 1982, the French-American-British (FAB) group proposed a classification for the MDS, based on morphological characteristics of peripheral blood and of the bone marrow. Later, cytogenetics proved to be a useful tool for the refinement of prognosis, through the use of the International Prognosis Score System (IPSS), as well as through evidence of clonality.

Composition of constitutive heterochromatin of Pseudonannolene strinatii Mauriès, 1974 (Diplopoda, Spirostreptida) analyzed by AT/CG specific fluorochromes

K. Agari Campos and Fontanetti, C. Silvia, Composition of constitutive heterochromatin of Pseudonannolene strinatii Mauriès, 1974 (Diplopoda, Spirostreptida) analyzed by AT/CG specific fluorochromes, vol. 4, pp. 765-770, 2005.

Triple staining with fluorochromes (DA/DAPI/CMA) and C-banding were used to characterize the composition of Pseudonannolene strinatii heterochromatin. C-banding showed C+ bands of different labeling intensity on chromosomes 1 and 2 in some cells. Fluorochrome staining revealed DAPI+ regions corresponding to the C-banding pattern, indicating that the heterochromatin of this species is abundant in AT-rich sequences.

Karyotypic studies of Cratylia argentea (Desv.) O. Kuntze and C. mollis Mart. ex Benth. (Fabaceae - Papilionoideae)

S. M. Vargas, Torres, G. A., Sobrinho, F. S., Pereira, A. V., and Davide, L. C., Karyotypic studies of Cratylia argentea (Desv.) O. Kuntze and C. mollis Mart. ex Benth. (Fabaceae - Papilionoideae), vol. 6, pp. 707-712, 2007.

Cratylia argentea and C. mollis (Fabaceae-Papilionoideae) are legume shrubs native to the Cerrado and Caatinga, respectively. Both species show great resistance to drought and high nutritive value, which makes them a valuable forage resource in tropical regions. Cytogenetic studies were carried out on accessions of C. argentea and C. mollis from Germplasm Banks of Embrapa Gado de Leite (Juiz de Fora, MG) and Embrapa Semi-Árido (Petrolina, PE), respectively.

The anticancer homeopathic composite “Canova Method” is not genotoxic for human lymphocytes in vitro

I. C. Seligmann, Lima, P. D. L., Cardoso, P. C. S., Khayat, A. S., Bahia, M. O., Buchi, Dde Freitas, Cabral, I. R., and Burbano, R. R., The anticancer homeopathic composite “Canova Method” is not genotoxic for human lymphocytes in vitro, vol. 2, pp. 223-228, 2003.

The Canova Methodâ (CM) is a homeopathic medicine indicated for the treatment of patients with cancer and for pathologies that involve a depressed immune system, such as AIDS. This product is composed of homeopathic dilutions of Aconitum napellus, Arsenicum album (arsenic trioxide), Bryonia alba, Lachesis muta venom and Thuya occidentalis. It stimulates the immune system by activating macrophages.

A technique to obtain fibroblast cells from skin biopsies of living bats (Chiroptera) for cytogenetic studies

R. Moratelli, Andrade, Cde Moraes, and de Armada, J. Luís Azev, A technique to obtain fibroblast cells from skin biopsies of living bats (Chiroptera) for cytogenetic studies, vol. 1, pp. 128-130, 2002.

We developed a procedure to obtain fibroblasts from bat skin. A small fragment of the ear is removed under ether anesthesia. This material is then cut up into small pieces and cultured in standard cell culture media. Very good quality chromosome preparations for cytogenetic studies are obtained in about three weeks. Secondary cultures can be used for other biological studies. This procedure does not require sacrificing the animals.

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