Damage model

Establishment of a blue light damage model of human retinal pigment epithelial cells in vitro

G. Su, Cai, S. J., Gong, X., Wang, L. L., Li, H. H., Wang, L. M., Su, G., Cai, S. J., Gong, X., Wang, L. L., Li, H. H., and Wang, L. M., Establishment of a blue light damage model of human retinal pigment epithelial cells in vitro, vol. 15, p. -, 2016.

To establish a blue-light damage model of human retinal pigment epithelium (RPE). Fourth-generation human RPE cells were randomly divided into two groups. In group A, cells were exposed to blue light (2000 ± 500 lux) for 0 (control), 3, 6, 9, and 12 h, and cell culture was stopped after 12 h. In group B, cells were exposed to blue light at the same intensity and time periods, but cell culture was stopped after 24 h. TdT-mediated dUTP nick-end labeling (TUNEL) assay was performed to determine the most suitable illuminating time with apoptotic index.

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