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Large-scale detection and application of expressed sequence tag single nucleotide polymorphisms in Nicotiana

Y. Wang, Zhou, D., Wang, S., and Yang, L., Large-scale detection and application of expressed sequence tag single nucleotide polymorphisms in Nicotiana, vol. 14, pp. 7793-7800, 2015.

Single nucleotide polymorphisms (SNPs) are widespread in the Nicotiana genome. Using an alignment and variation detection method, we developed 20,607,973 SNPs, based on the expressed sequence tag sequences of 10 Nicotiana species. The replacement rate was much higher than the transversion rate in the SNPs, and SNPs widely exist in the Nicotiana. In vitro verification indicated that all of the SNPs were high quality and accurate.

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