Diabetes

Identification of molecular markers related to human alveolar bone cells and pathway analysis in diabetic patients

X. Sun, Ren, Q. H., Bai, L., and Feng, Q., Identification of molecular markers related to human alveolar bone cells and pathway analysis in diabetic patients, vol. 14, pp. 13476-13484, 2015.

Alveolar bone osteoblasts are widely used in dental and related research. They are easily affected by systemic diseases such as diabetes. However, the mechanism of diabetes-induced alveolar bone absorption remains unclear. This study systematically explored the changes in human alveolar bone cell-related gene expression and biological pathways, which may facilitate the investigation of its mechanism. Alveolar bone osteoblasts isolated from 5 male diabetics and 5 male healthy adults were cultured. Total RNA was extracted from these cells and subjected to gene microarray analysis.

Aquaporin in the proliferation and apoptosis of diabetic myocardial cells

P. F. Zhao and Sun, M. J., Aquaporin in the proliferation and apoptosis of diabetic myocardial cells, vol. 14, pp. 17366-17372, 2015.

The aim of this study was to explore the effect of aquaporin on the molecular mechanism of human diabetic myocardial cell apoptosis. The methylthiazolyle tetrazolium assay was used to detect the inhibitory effect of different concentrations of aquaporin on cell growth. The rate of aquaporin-induced myocardial cell apoptosis was detected by flow cytometric analysis of Annexin V-fluorescein isothiocyanate/propidium iodide double-stained cells. We also attempted to quantify the expression of Bcl-2, Bax, caspase-3, and survivin in diabetic myocardial cells by western blot analysis.

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