Diabetes

Alleviation of streptozotocin-induced diabetes in nude mice by stem cells derived from human first trimester umbilical cord

M. Cao, Zhang, J. B., Dong, D. D., Mou, Y., Li, K., Fang, J., Wang, Z. Y., Chen, C., Zhao, J., and Yie, S. M., Alleviation of streptozotocin-induced diabetes in nude mice by stem cells derived from human first trimester umbilical cord, vol. 14, pp. 12505-12519, 2015.

Cells isolated from human first trimester umbilical cord perivascular layer (hFTM-PV) tissues display the pluripotent characteristics of stem cells. In this study, we examined whether hFTM-PV cells can differentiate into islet-like clusters (ILCs) in vitro, and whether transplantation of the hFTM-PV cells with and without differentiation in vitro can alleviate diabetes in nude mice. The hFTM-PV cells were differentiated into ILCs in vitro through a simple stepwise culture protocol.

Identification of molecular markers related to human alveolar bone cells and pathway analysis in diabetic patients

X. Sun, Ren, Q. H., Bai, L., and Feng, Q., Identification of molecular markers related to human alveolar bone cells and pathway analysis in diabetic patients, vol. 14, pp. 13476-13484, 2015.

Alveolar bone osteoblasts are widely used in dental and related research. They are easily affected by systemic diseases such as diabetes. However, the mechanism of diabetes-induced alveolar bone absorption remains unclear. This study systematically explored the changes in human alveolar bone cell-related gene expression and biological pathways, which may facilitate the investigation of its mechanism. Alveolar bone osteoblasts isolated from 5 male diabetics and 5 male healthy adults were cultured. Total RNA was extracted from these cells and subjected to gene microarray analysis.

Aquaporin in the proliferation and apoptosis of diabetic myocardial cells

P. F. Zhao and Sun, M. J., Aquaporin in the proliferation and apoptosis of diabetic myocardial cells, vol. 14, pp. 17366-17372, 2015.

The aim of this study was to explore the effect of aquaporin on the molecular mechanism of human diabetic myocardial cell apoptosis. The methylthiazolyle tetrazolium assay was used to detect the inhibitory effect of different concentrations of aquaporin on cell growth. The rate of aquaporin-induced myocardial cell apoptosis was detected by flow cytometric analysis of Annexin V-fluorescein isothiocyanate/propidium iodide double-stained cells. We also attempted to quantify the expression of Bcl-2, Bax, caspase-3, and survivin in diabetic myocardial cells by western blot analysis.

Effect of hypothalamic-pituitary-adrenal axis alterations on glucose and lipid metabolism in diabetic rats

M. W. Si, Yang, M. K., and Fu, X. D., Effect of hypothalamic-pituitary-adrenal axis alterations on glucose and lipid metabolism in diabetic rats, vol. 14, pp. 9562-9570, 2015.

This study investigated the relationship between alterations in the hypothalamic-pituitary-adrenal (HPA) axis function and glucose and lipid metabolism in diabetic rats. To accomplish this a diabetes model was established by jointly administering a long-term high-fat diet plus Streptozotocin (STZ; 50 mg/kg ip). The rats were randomly divided into four groups: 1) a normal control group, 2) a model group, 3) astragalus polysaccharide (APS) group, and 4) a metformin group. APS and metformin hydrochloride were administered intragastrically (100 mg∙kg-1d-1).

Changes and clinical significance of serum vaspin levels in patients with type 2 diabetes

L. Yang, Chen, S. J., Yuan, G. Y., Wang, D., and Chen, J. J., Changes and clinical significance of serum vaspin levels in patients with type 2 diabetes, vol. 14, pp. 11356-11361, 2015.

We investigated serum visceral adipose tissue-derived serpin (vaspin) levels in patients with normal glucose regulation and recently diagnosed type 2 diabetes (T2DM) and explored the association between vaspin and body mass index, age, gender, glucose, lipid metabolism, and insulin sensitivity. Fasting serum vaspin levels in 66 patients with T2DM and 48 normal subjects were detected using enzyme-linked immunosorbent assay. We found that serum vaspin levels in the DM group were 0.65 ± 0.13 mg/L in non-obese patients and 1.13 ± 0.25 mg/L in obese patients.

Subscribe to Diabetes