Differential expression

Effect of dual Bt-expression transformation vectors on transgene expression in tobacco

L. N. Xu, Dong, Y., Zhang, J., Wang, R. X., Liu, H. M., Yang, Q., Yang, M. S., Xu, L. N., Dong, Y., Zhang, J., Wang, R. X., Liu, H. M., Yang, Q., and Yang, M. S., Effect of dual Bt-expression transformation vectors on transgene expression in tobacco, vol. 15, p. -, 2016.

This study aimed to determine the influence of vector structure on dual Bt gene expression and establish an efficient expression vector using Cry1Ac and Cry3A genes. Four vectors (N4, N5, N10, and S23) were developed and used for genetic transformation of tobacco to obtain insect-resistant transgenic lines. The vectors were constructed using the MAR structure, applying different promoter and enhancer sequences, and changing the transgene open-reading frame sequence.

Comparative proteomic analysis reveals mite (Varroa destructor) resistance-related proteins in Eastern honeybees (Apis cerana)

T. Ji, Shen, F., Liu, Z., Yin, L., Shen, J., Liang, Q., and Luo, Y. X., Comparative proteomic analysis reveals mite (Varroa destructor) resistance-related proteins in Eastern honeybees (Apis cerana), vol. 14, pp. 10103-10118, 2015.

The mite (Varroa destructor) has become the greatest threat to apiculture worldwide. As the original host of the mite, Apis cerana can effectively resist the mite. An increased understanding of the resistance mechanisms of Eastern honeybees against V. destructor may help researchers to protect other species against these parasites. In this study, the proteomes of 4 Apis cerana colonies were analyzed using an isobaric tag for relative and absolute quantitation technology.

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