Differentiation

Assessment of genetic relationship in Persea spp by traditional molecular markers

J. C. Reyes-Alemán, Valadez-Moctezuma, E., Barrientos-Priego, A. F., Reyes-Alemán, J. C., Valadez-Moctezuma, E., Barrientos-Priego, A. F., Reyes-Alemán, J. C., Valadez-Moctezuma, E., and Barrientos-Priego, A. F., Assessment of genetic relationship in Persea spp by traditional molecular markers, vol. 15, p. -, 2016.

Currently, the reclassification of the genus Persea is under discussion with molecular techniques for DNA analysis representing an alternative for inter- and intra-specific differentiation. In the present study, the traditional random-amplified polymorphic DNA (RAPD) and the inter simple sequence repeat (ISSR) markers were used to determine the genomic relationship of different species and hybrids representative of the subgenera Eriodaphne and Persea in a population conserved in a germplasm bank.

Elevated TrkA receptor expression is associated with all-trans retinoic acid-induced neuroblastoma differentiation

Q. Gao, Chen, C. F., Dong, Q., Hou, L., Chen, X., Zhi, Y. L., Li, X., Lu, H. T., and Zhang, H. Y., Elevated TrkA receptor expression is associated with all-trans retinoic acid-induced neuroblastoma differentiation, vol. 14, pp. 13195-13202, 2015.

Neuroblastoma is the most common and one of the deadliest among pediatric tumors; however, a subset of infants with neuroblastoma display spontaneous regression. The mechanism of spontaneous regression remains to be elucidated. TrkA plays an essential role in the differentiation and functionality of neurons; abundant TrkA expression is associated with favorable prognosis of neuroblastoma. All-trans retinoic acid (ATRA), a first-line drug for acute promyelocytic leukemia (APL) treatment, has been shown to induce differentiation and inhibit cell growth.

Differentiation-inducing effects of betamethasone on human glioma cell line U251

T. Jin, Wang, Y. X., Fan, K., Tao, D. B., Dong, X., and Shen, J. S., Differentiation-inducing effects of betamethasone on human glioma cell line U251, vol. 14, pp. 7841-7849, 2015.

We studied the differentiation-inducing effect of beta­methasone on human glioma cell line U251 cultured in vitro, and the underlying mechanism. U251 cells were divided into two groups: con­trol group cells, cultured in Dulbecco’s Modified Eagle’s medium con­taining 10% fetal bovine serum; and medication group cells, treated with 15 μM betamethasone. Morphological cell changes were observed by inverted microscope, cell cycle changes were ascertained by flow cytometry, and vimentin expression was checked by immunocytochem­istry.

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