Dioecious

Identification of an SCAR marker related to female phenotype in Idesia polycarpa Maxim.

S. H. Wang, Li, Y., Li, Z. Q., Chang, L., and Li, L., Identification of an SCAR marker related to female phenotype in Idesia polycarpa Maxim., vol. 14, pp. 2015-2022, 2015.

Idesia polycarpa Maxim. is a dioecious species. Because of the lack of morphological and cytological methods available for identifying its sex during the long juvenile stage, the application of molecular markers in sex identification may facilitate sex determination in the seedling stage. The objective of this study was to use sequence-related amplified polymorphism to identify sex-linked markers in I. polycarpa and convert these markers into sequence-characterized amplified region markers, which are much easier to identify.

Identification of floral genes for sex determination in Calamus palustris Griff. by using suppression subtractive hybridization

C. Y. Ng, Wickneswari, R., and Choong, C. Y., Identification of floral genes for sex determination in Calamus palustris Griff. by using suppression subtractive hybridization, vol. 13, pp. 6037-6049, 2014.

Calamus palustris Griff. is an economically important dioecious rattan species in Southeast Asia. However, dioecy and onset of flowering at 3-4 years old render uncertainties in desired female:male seedling ratios to establish a productive seed orchard for this rattan species. We constructed a subtractive library for male floral tissue to understand the genetic mechanism for gender determination in C. palustris. The subtractive library produced 1536 clones with 1419 clones of high quality.

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