Disease resistance

In silico analysis of gene content in tomato genomic regions mapped to the Ty-2 resistance gene

Y. F. Liu, Wan, H. J., Wei, Y. P., Wang, R. Q., Ruan, M. Y., Ye, Q. J., Li, Z. M., Zhou, G. Z., Yao, Z. P., and Yang, Y. J., “In silico analysis of gene content in tomato genomic regions mapped to the Ty-2 resistance gene”, vol. 14, pp. 7947-7956, 2015.

Tomato yellow leaf curl virus is one of the main diseases affecting tomato production worldwide. Previous studies have shown that Ty-2 is an important resistance gene located between molecular markers C2_At2g28250 (82.3 cM) and T0302 (89.0 cM), and exhibits strong re­sistance to tomato yellow leaf curl virus in Asia. In this study, Ty-2 can­didate genes were subjected to bioinformatic analysis for the sequenced tomato genome.

Development of EST-SSR markers related to disease resistance and their application in genetic diversity and evolution analysis in Gossypium

B. H. Wang, Rong, P., Cai, X. X., Wang, W., Zhu, X. Y., Chen, C. J., Xu, Y. Y., Huang, X. J., Zhuang, Z. M., and Wang, C. B., “Development of EST-SSR markers related to disease resistance and their application in genetic diversity and evolution analysis in Gossypium”, vol. 14, pp. 10630-10644, 2015.

Cotton (Gossypium spp) is one of the most economically important crops that provide the world’s most widely used natural fiber. Diseases such as Fusarium wilt and particularly Verticillium wilt seriously affect cotton production, and thus breeding for disease resistance is one of the most important goals of cotton breeding programs. Currently, potential exists to improve disease resistance in cultivated cotton.

Identification and characterization of novel NBS-LRR resistance gene analogues from the pea

S. Djebbi, Bouktila, D., Makni, H., Makni, M., and Mezghani-Khemakhem, M., “Identification and characterization of novel NBS-LRR resistance gene analogues from the pea”, vol. 14, pp. 6419-6428, 2015.

Pea (Pisum sativum) is one of the most cultivated le­gumes in the world, and its yield and seed quality are affected by a variety of pathogens. In plants, NBS-LRR (nucleotide binding site-leucine-rich repeat) is the main class of disease resistance genes. Using degenerate primers deduced from conserved motifs in the NBS domain of known resistance genes, we identified 10 NBS sequences in three varieties of P. sativum.

Characterization of the β-1,3-glucanase gene in peanut (Arachis hypogaea L.) by cloning and genetic transformation

L. X. Qiao, Ding, X., Wang, H. C., Sui, J. M., and Wang, J. - S., “Characterization of the β-1,3-glucanase gene in peanut (Arachis hypogaea L.) by cloning and genetic transformation”, vol. 13, pp. 1893-1904, 2014.

Plant β-1,3-glucanases are commonly involved in disease resistance. This report describes the cloning and genetic transformation of a β-1,3-glucanase gene from peanut. The gene was isolated from both the genomic DNA and cDNA of peanut variety Huayu20 by polymerase chain reaction (PCR) and reverse transcription PCR (RT-PCR), respectively. The DNA sequence contained 1471 bp including two exons and one intron, and the coding sequence contained 1047 bp that coded for a 348-amino acid protein with a calculated molecular weight of 38.8 kDa.

Evaluation of powdery mildew-resistance of grape germplasm and rapid amplified polymorphic DNA markers associated with the resistant trait in Chinese wild Vitis

J. Zhang, Zhang, Y., Yu, H., and Wang, Y., “Evaluation of powdery mildew-resistance of grape germplasm and rapid amplified polymorphic DNA markers associated with the resistant trait in Chinese wild Vitis”, vol. 13, pp. 3599-3614, 2014.

The resistance of wild Vitis germplasm, including Chinese and American wild Vitis and Vitis vinifera cultivars, to powdery mildew (Uncinula necator Burr.) was evaluated for two consecutive years under natural conditions. Most of the Chinese and North American species displayed a resistant phenotype, whereas all of the European species were highly susceptible.

Soybean rust resistance sources and inheritance in the common bean (Phaseolus vulgaris L.)

T. L. P. O. Souza, Dessaune, S. N., Moreira, M. A., and Barros, E. G., “Soybean rust resistance sources and inheritance in the common bean (Phaseolus vulgaris L.)”, vol. 13, pp. 5626-5636, 2014.

Soybean rust (SBR), caused by the fungus Phakopsora pachyrhizi, has been reported in common bean (Phaseolus vulgaris L.) cultivars and elite lines that were infected under controlled and natural field conditions in South Africa, the United States, Argentina, and Brazil. Although SBR is currently not a top priority problem for the common bean crop, many bean breeders are concerned about this disease because of the high severity and virulence diversity of P. pachyrhizi and its broad host range. In this study, a set of 44 P.

Identification of quantitative trait loci for blast resistance in BC2F3 and BC2F5 advanced backcross families of rice

H. A. Rahim, Bhuiyan, M. A. R., Lim, L. S., Sabu, K. K., Saad, A., Azhar, M., and Wickneswari, R., “Identification of quantitative trait loci for blast resistance in BC2F3 and BC2F5 advanced backcross families of rice”, vol. 11, pp. 3277-3289, 2012.

Advanced backcross families derived from Oryza sativa cv MR219/O. rufipogon IRGC105491 were utilized for identification of quantitative trait loci (QTL) for blast resistance using simple sequence repeat markers. Two hundred and sixty-one BC2F3 families were used to construct a linkage map, using 87 markers, which covered 2375.2 cM of 12 rice chromosomes, with a mean density of 27.3 cM. The families were evaluated in a greenhouse for resistance to blast disease caused by pathotypes P7.2 and P5.0 of Magnaporthe oryzae.

Analysis of simple sequence repeat markers linked with blast disease resistance genes in a segregating population of rice (Oryza sativa)

S. Ashkani, Rafii, M. Y., Sariah, M., A. Akmar, S. Nor, Rusli, I., H. Rahim, A., and Latif, M. A., “Analysis of simple sequence repeat markers linked with blast disease resistance genes in a segregating population of rice (Oryza sativa)”, vol. 10, pp. 1345-1355, 2011.

Among 120 simple sequence repeat (SSR) markers, 23 polymorphic markers were used to identify the segregation ratio in 320 individuals of an F2 rice population derived from Pongsu Seribu 2, a resistant variety, and Mahsuri, a susceptible rice cultivar. For phenotypic study, the most virulent blast (Magnaporthe oryzae) pathotype, P7.2, was used in screening of F2 population in order to understand the inheritance of blast resistance as well as linkage with SSR markers.

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