DNA sequences

Genetic distances and phylogenetic trees of different Awassi sheep populations based on DNA sequencing

R. M. Al-Atiyat and Aljumaah, R. S., Genetic distances and phylogenetic trees of different Awassi sheep populations based on DNA sequencing, vol. 13, pp. 6557-6568, 2014.

This study aimed to estimate evolutionary distances and to reconstruct phylogeny trees between different Awassi sheep populations. Thirty-two sheep individuals from three different geographical areas of Jordan and the Kingdom of Saudi Arabia (KSA) were randomly sampled. DNA was extracted from the tissue samples and sequenced using the T7 promoter universal primer. Different phylogenetic trees were reconstructed from 0.64-kb DNA sequences using the MEGA software with the best general time reverse distance model. Three methods of distance estimation were then used.

Comparative analysis of noncoding sequences of orthologous bovine and human gene pairs

M. Nunes Miziara, Riggs, P. K., and M. Amaral, E. J., Comparative analysis of noncoding sequences of orthologous bovine and human gene pairs, vol. 3, pp. 465-473, 2004.

Genomic sequence comparison across species has enabled the elucidation of important coding and regulatory sequences encoded within DNA. Of particular interest are the noncoding regulatory sequences, which influence gene transcriptional and posttranscriptional processes. A phylogenetic footprinting strategy was employed to identify noncoding conservation patterns of 39 human and bovine orthologous genes. Seventy-three conserved noncoding sequences were identified that shared greater than 70% identity over at least 100 bp.

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