DpnI digestion

Highly efficient one-step PCR-based mutagenesis technique for large plasmids using high-fidelity DNA polymerase

H. Liu, Ye, R., and Wang, Y. Y., Highly efficient one-step PCR-based mutagenesis technique for large plasmids using high-fidelity DNA polymerase, vol. 14, pp. 3466-3473, 2015.

Gene mutation plays an important role in molecular biological studies. A highly efficient one-step polymerase chain reaction-based mutagenesis technique for site-directed mutagenesis was developed in this study. One complementary pair of primers was designed that contained the desired mutations in the middle of the primers. The amplification products of mutation were amplified using a high-fidelity DNA polymerase and the original plasmid templates were digested by DpnI.

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