Drought

Expression of the 2-dehydro-3-deoxyphosphooc­tonate aldolase (KdsA) gene in mulberry leaves (Morus alba L.) is down-regulated under high salt and drought stress

X. B. Yang, Wu, S. L., Zhu, D. P., Wu, H., Jiang, T., Qian, Y. H., and Jiao, F., “Expression of the 2-dehydro-3-deoxyphosphooc­tonate aldolase (KdsA) gene in mulberry leaves (Morus alba L.) is down-regulated under high salt and drought stress”, vol. 14, pp. 11955-11964, 2015.

The oligosaccharide 3-deoxy-d-manno-oct-2-ulosonic acid (Kdo) is a key component of lipopolysaccharide in Gram-negative bacteria, and is also part of the pectic polysaccharide rhamnogalacturonan (RG-II) of the plant cell wall. The enzyme KDO-8-phosphate synthase (KDO8Ps), encoded by the 2-dehydro-3-deoxyphosphooctonate aldolase (KdsA) gene, catalyzes the first step in the synthesis of Kdo. In this study, the complete coding sequence of the KdsA gene from mulberry leaves was cloned and the primary structure of KDO8Ps was deduced.

Genotypic variability for root/shoot parameters under water stress in some advanced lines of cotton (Gossypium hirsutum L.)

M. Riaz, Farooq, J., Sakhawat, G., Mahmood, A., Sadiq, M. A., and Yaseen, M., “Genotypic variability for root/shoot parameters under water stress in some advanced lines of cotton (Gossypium hirsutum L.)”, vol. 12, pp. 552-561, 2013.

Research pertaining to genetic variability parameters, heritability, and genotypic, phenotypic, simple, and environmental correlations for various seedling traits in five elite advanced cotton (Gossypium hirsutum L.) lines (FH-113, FH-114, FH-941, FH-942, and FH-2015) and one check (CIM-496) was carried out during October and November 2010 under greenhouse conditions at the Cotton Research Institute (Faisalabad, Pakistan). Material was raised in plastic tubes with a randomized complete block design replicated three times.

Comparative and joint analyses of gene expression profiles under drought and rewatering in Arabidopsis

Z. H. Xu and Wu, W. R., “Comparative and joint analyses of gene expression profiles under drought and rewatering in Arabidopsis”, vol. 12, pp. 3622-3629, 2013.

Drought is a major limiting factor in crop production. Rewatering is a process opposite to drought, allowing plants to recover to their normal physiological state. To understand more thoroughly the set of genes involved in plant response to drought, we comparatively and jointly analyzed the microarray data of drought and rewatering experiments in Arabidopsis. A total of 3833 differentially expressed genes (DEGs) were identified. Among them, ~74% were proven to be co-regulated by drought and rewatering.

Cloning and quantitative expression analysis of drought-induced genes in soybean

R. Stolf-Moreira, Medri, M. E., Neumaier, N., Lemos, N. G., Brogin, R. L., Marcelino, F. C., de Oliveira, M. C. N., Farias, J. R. B., Abdelnoor, R. V., and Nepomuceno, A. L., “Cloning and quantitative expression analysis of drought-induced genes in soybean”, vol. 9, pp. 858-867, 2010.

We determined the expression levels of DREB transcription factor (Gmdreb1) and of the genes Gmgols, Gmpip1b, Gmereb, and Gmdefensin in drought-tolerant (MG/BR46-Conquista) and drought-sensitive (BR16) genotypes of soybean, during drought. The trial was carried out in a controlled-environment chamber, set up to provide drought conditions. Sequences of Arabidopsis thaliana DREB-family proteins were used to build a phylogenetic tree through the alignment of the conserved regions near the AP2 domain.

Soybean physiology and gene expression during drought

R. Stolf-Moreira, Medri, M. E., Neumaier, N., Lemos, N. G., Pimenta, J. A., Tobita, S., Brogin, R. L., Marcelino-Guimarães, F. C., Oliveira, M. C. N., Farias, J. R. B., Abdelnoor, R. V., and Nepomuceno, A. L., “Soybean physiology and gene expression during drought”, vol. 9, pp. 1946-1956, 2010.

Soybean genotypes MG/BR46 (Conquista) and BR16, drought-tolerant and -sensitive, respectively, were compared in terms of morphophysiological and gene-expression responses to water stress during two stages of development. Gene-expression analysis showed differential responses in Gmdreb1a and Gmpip1b mRNA expression within 30 days of water-deficit initiation in MG/BR46 (Conquista) plants. Within 45 days of initiating stress, Gmp5cs and Gmpip1b had relatively higher expression.

Cassava genetic resources and their utilization for breeding of the crop

N. M. A. Nassar, “Cassava genetic resources and their utilization for breeding of the crop”, vol. 6. pp. 1151-1168, 2007.

Wild cassava relatives are perennials and vary in growth pattern from nearly acaulescent subshrubs to small trees. They have been used as a source of useful characters such as high protein content, apomixis, resistance to mealybug and mosaic disease, and tolerance to drought. Indigenous clones are a potential source of β-carotene and lycopene. Apomixis genes have been transferred to the crop successfully through interspecific hybridization, and apomictic clones arising from these hybrids are now being grown at the Universidade de Brasília.

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