Drug resistance

Development of a new amplification-refractory mutation system for detection of a single nucleotide polymorphism linked to drug resistance in Ancylostoma caninum

L. F. Furtado and Rabelo, É. M., Development of a new amplification-refractory mutation system for detection of a single nucleotide polymorphism linked to drug resistance in Ancylostoma caninum, vol. 14, pp. 5103-5111, 2015.

Single nucleotide polymorphisms at codons 167, 198, and 200 in the β-tubulin isotype 1 gene have been associated with benz­imidazole resistance. Until now, the only mutation observed in Ancy­lostoma caninum was at codon 200 of this gene. However, the standard­ized methodologies used to detect mutations in this species are faulty. The objective of this study was to standardize a molecular technique based on amplification-refractory mutation system-polymerase chain reaction (ARMS-PCR) for detecting the mutation at codon 200 in the A.

Autophagy in drug resistance of the multiple myeloma cell line RPMI8226 to doxorubicin

Y. - Z. Pan, Wang, X., Bai, H., Wang, C. - B., Zhang, Q., and Xi, R., Autophagy in drug resistance of the multiple myeloma cell line RPMI8226 to doxorubicin, vol. 14, pp. 5621-5629, 2015.

We investigated the effect of autophagy on drug resistance of multiple myeloma (MM) to doxorubicin (DOX). A DOX-resistant MM cell line (RPMI8226/DOX) was developed by progressively increasing the DOX concentration gradient. The drug resistance index was determined using the MTT method. Transmission electron microscopy, anti-light chain 3-fluorescein isothiocyanate immunofluorescence, and Western blotting were used to detect autophagy of MM cells.

Relationship between RFC gene expression and intracellular drug concentration in methotrexate-resistant osteosarcoma cells

J. J. Wang and Li, G. J., Relationship between RFC gene expression and intracellular drug concentration in methotrexate-resistant osteosarcoma cells, vol. 13, pp. 5313-5321, 2014.

Osteosarcoma is a primary malignant tumor in adolescents, associated with high mortality and morbidity. The high-dose methotrexate (MTX) chemotherapy used to treat this disease may induce primary or secondary drug resistance, resulting in a reduced effect of comprehensive treatment. In this study, the relationship between reduced folate carrier (RFC) gene expression and intracellular drug concentration in MTX-resistant osteosarcoma cells (Saos-2) was investigated. MTX-resistant human osteosarcoma cells (Saos-2/MTX2.2, Saos-2/MTX4.4) were prepared.

Drug resistance analysis of bacterial strains isolated from burn patients

L. F. Wang, Li, J. L., Ma, W. H., and Li, J. Y., Drug resistance analysis of bacterial strains isolated from burn patients, vol. 13, pp. 9727-9734, 2014.

This study aimed to analyze the spectrum and drug resistance of bacteria isolated from burn patients to provide a reference for rational clinical use of antibiotics. Up to 1914 bacterial strain specimens isolated from burn patients admitted to hospital between 2001 and 2010 were subjected to resistance monitoring by using the K-B paper disk method. Retrospective analysis was performed on drug resistance analysis of burn patients. The top eight bacterium strains according to detection rate.

Multidrug resistance gene and its relationship to ulcerative colitis and immune status of ulcerative colitis

Y. J. Zhang, Xu, J. J., Wang, P., and Wang, J. L., Multidrug resistance gene and its relationship to ulcerative colitis and immune status of ulcerative colitis, vol. 13, pp. 10837-10851, 2014.

We examined the relationship among the multidrug resistance (MDR1) gene product P-glycoprotein (P-gp), ulcerative colitis, and immune status under ulcerative colitis. MDR1 P-gp expression and interleukin-8 levels in ulcerative colitis were determined using immunohistochemistry and a double-antibody sandwich avidin-biotin complex-enzyme-linked immunosorbent assay, respectively.

Transporters in the Paracoccidioides brasiliensis transcriptome: insights on drug resistance

Cda Silva Costa, Albuquerque, F. Caixeta, Andrade, R. Vieira, de Oliveira, G. Camilo, de Almeida, M. Fernandes, Brigido, Mde Macedo, Brigido, Mde Macedo, Maranhão, A. Queiróz, and Maranhão, A. Queiróz, Transporters in the Paracoccidioides brasiliensis transcriptome: insights on drug resistance, vol. 4, pp. 390-408, 2005.

In the struggle for life, the capacity of microorganisms to synthesize and secrete toxic compounds (inhibiting competitors) plays an important role in successful survival of these species. This ability must come together with the capability of being unaffected by these same compounds. Several mechanisms are thought to avoid the toxic effects. One of them is toxin extrusion from the intracellular environment to the outside vicinity, using special transmembrane proteins, referred to as transporters.

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