Embryo development

Construction and analysis of a subtractive cDNA library of early embryonic development in duck

Y. L. Liu, Zhong, L. X., Li, J. J., Shen, J. D., Wang, D. Q., Tao, Z. R., Shi, F. X., and Lu, L. Z., “Construction and analysis of a subtractive cDNA library of early embryonic development in duck”, vol. 12, pp. 2234-2247, 2013.

Several studies have documented the process of early embryonic development in poultry; however, the molecular mechanisms underlying its developmental regulation are poorly understood, particularly in ducks. In this study, we analyzed differential gene expression of embryos 6 and 25 h following oviposition to determine which genes regulate the early developmental stage in ducks. Among 216 randomly selected clones, 39 protein-encoding cDNAs that function in metabolism, transcription, transportation, proliferation/apoptosis, cell cycle, cell adhesion, and methylation were identified.

Molecular characterization of cathepsin L cDNA and its expression during oogenesis and embryogenesis in the oriental river prawn Macrobrachium nipponense (Palaemonidae)

W. Zhao, Chen, L., Zhang, F., Wu, P., Li, E., and Qin, J., “Molecular characterization of cathepsin L cDNA and its expression during oogenesis and embryogenesis in the oriental river prawn Macrobrachium nipponense (Palaemonidae)”, vol. 12, pp. 5215-5225, 2013.

We identified the cDNA sequence of cathepsin L (CatL) in Macrobrachium nipponense, designated as MnCatL, for the first time. The MnCatL cDNA, isolated from the ovary, was 1710 bp in length, containing a 31-bp 5'-untranslated region, a 650-bp 3'-untranslated region, and an open reading frame of 1029 bp, encoding a 342-amino acid polypeptide with a predicted molecular mass of 37.7 kDa. The polypeptide is composed of an 18-amino acid signal peptide, a 106-amino acid propeptide and a 218-amino acid mature peptide.

RNA-binding motif protein RBM22 is required for normal development of zebrafish embryos

F. He, Wang, C. - T., and Gou, L. - T., “RNA-binding motif protein RBM22 is required for normal development of zebrafish embryos”, vol. 8, pp. 1466-1473, 2009.

RBM22 is a newly discovered RNA-binding motif protein, belonging to the SLT11 family; it has been reported to be involved in pre-splicesome assembly and to interact with the Ca2+-signaling protein ALG-2. However, previous studies have not demonstrated whether its expression is essential for early embryogenesis in vertebrates. We utilized zebrafish as a developmental model to study the role of RBM22 during embryogenesis. The aligned protein sequences of RBM22 were found to be highly conserved.

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