Endophytic bacterium

Diversity of endophytic bacteria in Brazilian sugarcane

G. S. Magnani, Didonet, C. M., Cruz, L. M., Picheth, C. F., Pedrosa, F. O., and Souza, E. M., Diversity of endophytic bacteria in Brazilian sugarcane, vol. 9, pp. 250-258, 2010.

Endophytic bacteria live inside plant tissues without causing disease. Studies of endophytes in sugarcane have focused on the isolation of diazotrophic bacteria. We examined the diversity of endophytic bacteria in the internal tissues of sugarcane stems and leaves, using molecular and biochemical methods. Potato-agar medium was used to cultivate the endophytes; 32 isolates were selected for analysis. DNA was extracted and the 16S rRNA gene was partially sequenced and used for molecular identification.

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