Escherichia coli O157:H7

A duplex SYBR Green I real-time quantitative PCR assay for detecting Escherichia coli O157:H7

X. Yang, Cheng, H. W., Chen, L., Zhao, J., Chang, H. T., Wang, X. W., Liu, H. Y., Yao, H. X., Zhang, L. X., and Wang, C. Q., A duplex SYBR Green I real-time quantitative PCR assay for detecting Escherichia coli O157:H7, vol. 12, pp. 4836-4845, 2013.

PCR and hybridization assays are widely used for the detection and identification of Escherichia coli serogroups and serotypes. We used these techniques for the detection of E. coli O157:H7, a dominant serogroup among E. coli strains that are considered major public health problems worldwide. We developed a quantitative PCR assay using SYBR Green I, based on the published sequences of the rfbE and fliC genes from E. coli O157:H7. This method detected the E.

Subscribe to Escherichia coli O157:H7