EST

Development of polymorphic microsatellite markers based on expressed sequence tags in Populus cathayana (Salicaceae)

Z. Z. Tian, Zhang, F. Q., Cai, Z. Y., Chen, S. L., Tian, Z. Z., Zhang, F. Q., Cai, Z. Y., and Chen, S. L., “Development of polymorphic microsatellite markers based on expressed sequence tags in Populus cathayana (Salicaceae)”, vol. 15, p. -, 2016.

Populus cathayana occupies a large area within the northern, central, and southwestern regions of China, and is considered to be an important reforestation species in western China. In order to investigate the population genetic structure of this species, 10 polymorphic microsatellite loci were identified based on expressed sequence tags from de novo sequencing on the Illumina HiSeq 2000 platform. All microsatellite primers were tested on 48 P. cathayana individuals from four locations on the Qinghai-Tibet Plateau.

Construction of a cDNA library and preliminary analysis of expressed sequence tags in Piper hainanense

R. Fan, Ling, P., Hao, C. Y., Li, F. P., Huang, L. F., Wu, B. D., and Wu, H. S., “Construction of a cDNA library and preliminary analysis of expressed sequence tags in Piper hainanense”, vol. 14, pp. 12733-12745, 2015.

Black pepper is a perennial climbing vine. It is widely cultivated because its berries can be utilized not only as a spice in food but also for medicinal use. This study aimed to construct a standardized, high-quality cDNA library to facilitated identification of new Piper hainanense transcripts. For this, 262 unigenes were used to generate raw reads. The average length of these 262 unigenes was 774.8 bp. Of these, 94 genes (35.9%) were newly identified, according to the NCBI protein database. Thus, identification of new genes may broaden the molecular knowledge of P.

Large-scale detection and application of expressed sequence tag single nucleotide polymorphisms in Nicotiana

Y. Wang, Zhou, D., Wang, S., and Yang, L., “Large-scale detection and application of expressed sequence tag single nucleotide polymorphisms in Nicotiana”, vol. 14, pp. 7793-7800, 2015.

Single nucleotide polymorphisms (SNPs) are widespread in the Nicotiana genome. Using an alignment and variation detection method, we developed 20,607,973 SNPs, based on the expressed sequence tag sequences of 10 Nicotiana species. The replacement rate was much higher than the transversion rate in the SNPs, and SNPs widely exist in the Nicotiana. In vitro verification indicated that all of the SNPs were high quality and accurate.

Computational identification and characterization of conserved miRNAs and their target genes in beet (Beta vulgaris)

J. L. Li, Cui, J., and Cheng, D. Y., “Computational identification and characterization of conserved miRNAs and their target genes in beet (Beta vulgaris)”, vol. 14, pp. 9103-9108, 2015.

Highly conserved endogenous non-coding microRNAs (miRNAs) play important roles in plants and animals by silencing genes via destruction or blocking of translation of homologous mRNA. Sugar beet, Beta vulgaris, is one of the most important sugar crops in China, with properties that include wide adaptability and strong tolerance to salinity and impoverished soils. Seedlings of B.

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