EST-PCR marker

Expressed sequence tag-PCR markers for identification of alien barley chromosome 2H in wheat

M. J. Wang, Zou, H. D., Lin, Z. S., Wu, Y., Chen, X., and Yuan, Y. P., Expressed sequence tag-PCR markers for identification of alien barley chromosome 2H in wheat, vol. 11, pp. 3452-3463, 2012.

We developed EST-PCR markers specific to barley chromosome 2H, for the purpose of effectively tracing alien chromosomes or chromosome parts in the wheat genetic background. The target alien chromosome 2H confers high resistance to pre-harvest sprouting, which is a worldwide natural disaster in wheat. A total of 120 primer pairs were selected by combining the wheat group 2 chromosomes of the EST database and the genome sequences of the new model plant Brachypodium distachyon.

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