Until recently, Fenneropenaeus penicillatus was considered a commercial shrimp species. However, in 2005, it was included on the Red List as an endangered species by the Chinese government. In this study, 19 new microsatellite markers in F. penicillatus were developed and tested in samples of 32 wild individuals from Nanao, China. Twelve loci were polymorphic and 7 were monomorphic. Of the 12 polymorphic loci, the number of alleles per locus ranged from 3 to 6, with an average of 4.42 alleles per locus.
Thirteen new polymorphic microsatellite markers in Fenneropenaeus penicillatus were isolated and characterized. The polymorphism of the thirteen microsatellite markers was tested by 30 individuals from Lianjiang, China. It showed that the number of alleles per locus ranged from 3 to 6 and the Polymorphism Information Content (PIC) was from 0.324 to 0.706. The observed and expected heterozygosities were 0.3217-0.8023 and 0.1977-0.6783, respectively. Only one loci (LJ-19) deviated significantly from Hardy-Weinberg equilibrium (HWE) (P 0.00385).
Fenneropenaeus penicillatus, with high protein and low fat, is a commercially important aquatic product in China. Microsatellite loci were developed according to the protocol of fast isolation by amplified fragment length polymorphism of sequences containing repeats. Eight new polymorphic microsatellite markers for F. penicillatus were identified, and 32 wild individuals were used to evaluate the degree of polymorphism of these markers. The polymorphism information content ranged from 0.2703 to 0.7598, and the number of alleles per locus varied from 3 to 6.