Expression and purification of GST-FHL2 fusion protein

H. Yu, Ma, Q., Lin, J., Sun, Y. F., and Zheng, F., Expression and purification of GST-FHL2 fusion protein, vol. 12, pp. 6372-6378, 2013.

Escherichia coli is the most widely used host for the production of recombinant proteins. However, most eukaryotic proteins are typically obtained as insoluble, misfolded inclusion bodies that need solubilization and refolding. The interactions between human FHL2 protein and many types of proteins, including structural proteins, kinases, and several classes of transcription factor, have been found to have important roles in a variety of fundamental processes, including arrhythmia, hypertrophy, atherosclerosis, and angiogenesis.

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