FIASCO

Isolation and characterization of new microsatellite loci in Fenneropenaeus penicillatus

B. B. Li, Li, Z. B., Ning, Y. F., Shangguan, J. B., Huang, Y. S., Yuan, Y., Mao, X. Q., Li, B. B., Li, Z. B., Ning, Y. F., Shangguan, J. B., Huang, Y. S., Yuan, Y., Mao, X. Q., Li, B. B., Li, Z. B., Ning, Y. F., Shangguan, J. B., Huang, Y. S., Yuan, Y., and Mao, X. Q., Isolation and characterization of new microsatellite loci in Fenneropenaeus penicillatus, vol. 15, p. -, 2016.

Fenneropenaeus penicillatus is one of the major economic shrimp species in China. In this study, 14 novel microsatellite loci were developed using the fast isolation protocol with amplified fragment length polymorphism of sequences containing repeats (FIASCO). Polymorphisms were tested in 30 individuals from a single-wild population. The results showed that the number of alleles at each locus ranged from two to four, and the polymorphism information content varied from 0.314 to 0.692.

Isolation and characterization of microsatellite loci in Branchiostoma belcheri Gray (Amphioxus)

Z. B. Li, Huang, Y. S., Shangguan, J. B., Ning, Y. F., Yi, Y., and Dai, G., Isolation and characterization of microsatellite loci in Branchiostoma belcheri Gray (Amphioxus), vol. 14, pp. 10224-10227, 2015.

Branchiostoma belcheri Gray is a second-class, nationally protected protochordate in China. We developed 10 novel polymorphic sites in B. belcheri, which were examined using a population of 30 wild individuals from Xiamen, China. The polymorphism information content ranged between 0.141 and 0.681, and the number of alleles varied from 2 to 5. The expected and observed heterozygosities varied between 0.1528 and 0.6920, and between 0.1429 and 0.5000, respectively. These novel microsatellite markers will facilitate the genetic analysis and protection of wild B.

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