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Construction of a molecular database for soybean cultivar identification in Brazil

M. B. Oliveira, Vieira, E. S. N., and Schuster, I., Construction of a molecular database for soybean cultivar identification in Brazil, vol. 9, pp. 705-720, 2010.

The narrow genetic base of soybean makes cultivar characterization based on morphological descriptors difficult; this characterization is mainly done for registration and protection. Correct characterization of cultivars could be achieved through molecular markers, since the frequencies of each allele in the population are known. Consequently, we developed a molecular characterization method and initiated the construction of a molecular database for soybean cultivar identification. Thirty-two soybean cultivars were analyzed with 48 fluorescent-labeled microsatellite markers.

DNA profiling of sugarcane genotypes using randomly amplified polymorphic DNA

S. Tabasum, Khan, F. A., Nawaz, S., Iqbal, M. Z., and Saeed, A., DNA profiling of sugarcane genotypes using randomly amplified polymorphic DNA, vol. 9, pp. 471-483, 2010.

DNA profiles of 40 sugarcane genotypes were constructed with 30 RAPD markers. Sugarcane genotypes of both Saccharum officinarum and S. barberi were included in this study. Multiple alleles were detected from each RAPD; there was a high level of polymorphism. On average, 7.93 alleles were produced per primer, giving a total of 238 alleles. The genetic distances between these genotypes were assessed with the POPGENE DNA sequence analysis software. A dendrogram was constructed from these data; cultivated species of sugarcane formed clusters with S.

Genetic studies of “noble cane” for identification and exploitation of genetic markers

S. Nawaz, Khan, F. A., Tabasum, S., Iqbal, M. Z., and Saeed, A., Genetic studies of “noble cane” for identification and exploitation of genetic markers, vol. 9, pp. 1011-1022, 2010.

Forty genotypes (clones) of sugarcane, including elite lines, commercial cultivars of Saccharum officinarum and clones of S. barberi were fingerprinted with 50 SSR markers using a PCR-based marker assay. Nei’s genetic distances for SSR data were determined and relationships between accessions were portrayed graphically in the form of a dendrogram. Genetic distance values ranging from 0.60 to 1.11 were observed among the 40 sugarcane accessions. The shortest genetic distance of 0.60 was seen between genotypes US-804 and US-130.

Phylogenetic relationships among Saccharum clones in Pakistan revealed by RAPD markers

S. Nawaz, Khan, F. A., Tabasum, S., Zakria, M., Saeed, A., and Iqbal, M. Z., Phylogenetic relationships among Saccharum clones in Pakistan revealed by RAPD markers, vol. 9, pp. 1673-1682, 2010.

Forty sugarcane genotypes (clones), including elite lines, commercial cultivars of Saccharum officinarum and S. barberi clones, were fingerprinted with 30 RAPD markers, using a PCR-based marker assay. The genetic distance for RAPD data was determined according to Nei, and relationships between accessions were graphed in a dendrogram. Genetic distance values ranging from 16.2 to 86.3% were observed among the 40 sugarcane accessions. The lowest genetic distance was found between genotypes US-406 and US-186.

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