Fluorescence in situ hybridization

Identification of the origin of marker chromosomes by two-color fluorescence in situ hybridization and polymerase chain reaction in azoospermic patients

C. L. Wei, Cheng, J. L., Yang, W. C., Li, L. Y., Cheng, H. C., and Fu, J. J., Identification of the origin of marker chromosomes by two-color fluorescence in situ hybridization and polymerase chain reaction in azoospermic patients, vol. 14, pp. 14488-14495, 2015.

Y chromosomal microdeletions at the azoospermia factor locus and chromosome abnormalities have been implicated as the major causes of idiopathic male infertility. A marker chromosome is a structurally abnormal chromosome in which no part can be identified by cytogenetics. In this study, to identify the origin of the marker chromosomes and to perform a genetic diagnosis of patients with azoospermia, two-color fluorescence in situ hybridization (FISH) and polymerase chain reaction (PCR) techniques were carried out.

Replication timing regulation in adults with chromosomal balance rearrangements

Y. Xie, Wu, J., Lin, S., Zhou, Y., Fang, Q., and Sun, X., Replication timing regulation in adults with chromosomal balance rearrangements, vol. 14, pp. 7833-7840, 2015.

The alternative forms of the alleles in biallelic genes display a synchronous pattern of replication that is different from genes subjected to monoallelic expression, which exhibit an asynchronous mode of replication. The present study sought to gain insight into changes in the allele-specific replication timing in phenotypically normal humans with balanced chromosomal rearrangements, and to investigate the potential mechanism for chromosomal rearrangements.

Cytogenetic characterization of species of the family Heptapteridae (Teleostei: Siluriformes) from the Cuiabá and Ivaí River Basins, Brazil

L. M. Barbosa, Ferreira, G. E. B., Prizon, A. C., Portela-Castro, A. L. B., and Martins-Santos, I. C., Cytogenetic characterization of species of the family Heptapteridae (Teleostei: Siluriformes) from the Cuiabá and Ivaí River Basins, Brazil, vol. 14, pp. 8640-8649, 2015.

We cytogenetically characterized three species of Hep­tapteridae (Pimelodella sp, Pimelodella taenioptera, and Imparfinis schubarti) by investigating the distribution of constitutive heterochro­matin and nucleolar organizer regions by silver nitrate impregnation (Ag-NOR) and fluorescence in situ hybridization. Pimelodella sp showed had a diploid number (2n) = 46 chromosomes, 26m + 10sm + 10st, and FN = 92; P. taenioptera, 2n = 52 chromosomes, 26m + 22sm + 4st, and FN = 104; and I.

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