Fluorescence in situ hybridization

Clinical outcome in chronic myeloid leukemia after allogeneic hematopoietic stem cell transplantation: the experience of the Bone Marrow Transplantation Unit of FUNFARME/BRAZIL using FISH

C. B. Vendrame-Goloni, Carvalho-Salles, A. B., Ruiz, M. A., O. Júnior, R., Varella-Garcia, M., and Fett-Conte, A. C., Clinical outcome in chronic myeloid leukemia after allogeneic hematopoietic stem cell transplantation: the experience of the Bone Marrow Transplantation Unit of FUNFARME/BRAZIL using FISH, vol. 7, pp. 417-423, 2008.

Investigation of the efficacy of allogeneic hematopoietic stem cell transplantation (allo-HSCT) in chronic myeloid leukemia patients is essential to predict prognosis and survival. In 20 patients treated at the Bone Marrow Transplantation Unit of São José do Rio Preto (São Paulo, Brazil), we used fluorescence in situ hybridization (FISH) to investigate the frequency of cells with BCR/ABL rearrangement at diagnosis and at distinct intervals after allo-HSCT until complete cytogenetic remission (CCR).

Fluorescence in situ hybridization analysis with subtelomere specific probes (12pter-15qter) showed no differences in deletion patterns between normotensive and essential hypertension

S. T. Onrat and Tomatir, A. G., Fluorescence in situ hybridization analysis with subtelomere specific probes (12pter-15qter) showed no differences in deletion patterns between normotensive and essential hypertension, vol. 7, pp. 762-771, 2008.

Telomere biology is intimately linked to the genetic/environmental etiology of cardiovascular and metabolic diseases and telomere shortening is emerging as an important biomarker disease. The relationship between subtelomeric deletions and genetic hypertension was examined. Fluorescence in situ hybridization was used to directly assess whether there is a loss or gain of subtelomere copy number.

Identification of PML/RARα fusion gene transcripts that showed no t(15;17) with conventional karyotyping and fluorescent in situ hybridization

A. Choughule, Polampalli, S., Amre, P., Shinde, S., Banavali, S., Prabhash, K., Nair, R., Subramanian, P. G., Gujral, S., and Parikh, P. M., Identification of PML/RARα fusion gene transcripts that showed no t(15;17) with conventional karyotyping and fluorescent in situ hybridization, vol. 8, pp. 1-7, 2009.

Acute promyelocytic leukemia (APL) is characterized by a reciprocal translocation, t(15;17)(q22;q11-21), resulting in the fusion of the promyelocytic leukemia (PML) and retinoic acid receptor alpha (RARα) genes. Using conventional cytogenetic methods, these translocations are normally detected in about 70-90% of patients; most negative results are due to technical problems or cryptic variants.

New insights into telomeric DNA sequence (TTAGGG)n location in bat chromosomes

K. C. Faria, Marchesin, S. R. C., Moreira, P. R. L., Beguelini, M. R., and Morielle-Versute, E., New insights into telomeric DNA sequence (TTAGGG)n location in bat chromosomes, vol. 8. pp. 1079-1084, 2009.

Molossidae species, Cynomops abrasus (2n = 34, fun­damental number, FN = 64), Eumops auripendulus (2n = 42, FN = 62), Molossus rufus (2n = 48, FN = 64), Molossops temminckii (2n = 48, FN = 64), and Nyctinomops laticaudatus (2n = 48, FN = 64), and Phyl­lostomidae species, Phyllostomus discolor (2n = 32, FN = 60), have karyotypes with different chromosome and fundamental numbers, dif­ferent localization of constitutive heterochromatin, and different num­bers and location of nucleolar organizer regions (NORs).

Uses and limitations of two molecular cytogenetic techniques for the study of arrested embryos obtained through assisted reproduction technology

M. C. Muhlmann, Laudicina, A. O., Perandones, C., Bertolino, M. V., Marazzi, A., Quintans, C. J., Donaldson, M., Bozzo, W., and Pasqualini, S., Uses and limitations of two molecular cytogenetic techniques for the study of arrested embryos obtained through assisted reproduction technology, vol. 4, pp. 143-151, 2005.

We studied chromosomal abnormalities in arrested embryos produced by assisted reproductive technology with fluorescence in situ hybridization (FISH) and comparative genomic hybridization (CGH) in order to determine the best technique for evaluating chromosomal aneusomies to be implemented in different situations. We examined individual blastomeres from arrested embryos by FISH and arrested whole embryos by CGH. All of the 10 FISH-analyzed embryos gave results, while only 7 of the 30 embryos analyzed by CGH were usable.

Molecular cytogenetics in metaphase and interphase cells for cancer and genetic research, diagnosis and prognosis. Application in tissue sections and cell suspensions

M. Mühlmann and Mühlmann, M., Molecular cytogenetics in metaphase and interphase cells for cancer and genetic research, diagnosis and prognosis. Application in tissue sections and cell suspensions, vol. 1. pp. 117-127, 2002.

As the pioneer among molecular cytogenetics techniques, fluorescence in situ hybridization (FISH) allows identification of specific sequences in a structurally preserved cell, in metaphase or interphase. This technique, based on the complementary double-stranded nature of DNA, hybridizes labeled specific DNA (probe). The probe, bound to the target, will be developed into a fluorescent signal.

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