Fusion protein

Protein transduction domain-hA20 fusion protein protects endothelial cells against high glucose-induced injury

C. L. Hou, Huang, Q., Wei, Y., Zhang, W., Mi, J. H., Ying, D. J., and Zhou, Z. H., Protein transduction domain-hA20 fusion protein protects endothelial cells against high glucose-induced injury, vol. 11, pp. 1899-1908, 2012.

We constructed a plasmid containing a protein transduction domain (PTD) and a human A20 (hA20) gene fragment; the fusion protein was obtained by highly expressing this plasmid in the yeast Pichia pastoris GS115. The plasmid was obtained by adding 9xArg and EcoRІ recognition sites to the end of the primer, and 6xHis-Tag and NotІ recognition sites to its end.

Coexpression of interleukin-6 and -2 from giant panda in Escherichia coli and the biological activity of the fusion protein

Y. Yi, Nian, Y. - Y., Ji, H. - W., Zhang, H., Zhu, L., and Xu, Z. - W., Coexpression of interleukin-6 and -2 from giant panda in Escherichia coli and the biological activity of the fusion protein, vol. 12, pp. 1987-1995, 2013.

To construct a fusion cytokine protein with more and stronger bioactivities to enhance the immunity of the cytokine alone, we expressed interleukin (IL)-6/(IL)-2 from giant panda (Ailuropoda melanoleuca) in Escherichia coli as a 59.4-kDa fusion protein. Subsequently, the inclusion bodies were solubilized with 8 M urea and applied onto a Ni-nitrilotriacetic acid column. The final production of IL-6/IL-2 reached 6 mg/L in soluble form, and the purified final product was >96% pure.

Expression and purification of GST-FHL2 fusion protein

H. Yu, Ma, Q., Lin, J., Sun, Y. F., and Zheng, F., Expression and purification of GST-FHL2 fusion protein, vol. 12, pp. 6372-6378, 2013.

Escherichia coli is the most widely used host for the production of recombinant proteins. However, most eukaryotic proteins are typically obtained as insoluble, misfolded inclusion bodies that need solubilization and refolding. The interactions between human FHL2 protein and many types of proteins, including structural proteins, kinases, and several classes of transcription factor, have been found to have important roles in a variety of fundamental processes, including arrhythmia, hypertrophy, atherosclerosis, and angiogenesis.

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