Gene expression

Molecular characterization of the myosatin gene and the effect of fasting on its expression in Chinese perch (Siniperca chuatsi)

L. Liu, Li, Y. L., Xu, S. D., Wang, K. Z., Wu, P., Chu, W. Y., Wang, X. Q., Liu, L., Li, Y. L., Xu, S. D., Wang, K. Z., Wu, P., Chu, W. Y., and Wang, X. Q., “Molecular characterization of the myosatin gene and the effect of fasting on its expression in Chinese perch (Siniperca chuatsi)”, vol. 15, p. -, 2016.

Myostatin (MSTN) is an important member of the transforming growth factor-β (TGF-β) superfamily and is a muscle growth inhibitor. In the present study, we cloned the Chinese perch MSTN cDNA sequence and analyzed its expression patterns under various conditions. The MSTN full cDNA sequence was 3347 bp long, including an open-reading frame of 1131 bp, which encoded 376 amino acids.

Association of CD36 expression and polymorphism with serum biochemical indices in Cherry Valley duck

D. D. Wang, Li, W. G., Zhang, Y. Y., Wang, D. D., Li, W. G., and Zhang, Y. Y., “Association of CD36 expression and polymorphism with serum biochemical indices in Cherry Valley duck”, vol. 15, p. -, 2016.

Cluster of differentiation 36 (CD36) plays a crucial role in lipid sensing, innate immunity, atherogenesis, and glycolipid metabolism. This aims of this study were to delineate the CD36 mRNA expression profile in 16 duck tissues using relative quantitative real-time PCR and to screen single nucleotide polymorphisms (SNPs) in the duck CD36 gene by PCR-single strand conformation polymorphism and DNA direct sequencing. In addition, this study investigated CD36 gene expression, genetic variation, and their effect on serum biochemical indices in duck.

Dietary glycerol for quail: association between productive performance and COX III mRNA expression

S. C. C. Silva, Gasparino, E., Batista, E., Tanamati, F., Vesco, A. P. D., Lala, B., de Oliveira, D. P., Silva, S. C. C., Gasparino, E., Batista, E., Tanamati, F., Vesco, A. P. D., Lala, B., and de Oliveira, D. P., “Dietary glycerol for quail: association between productive performance and COX III mRNA expression”, vol. 15, p. -, 2016.

This study was carry out to evaluate mRNA expression of mitochondrial cytochrome c oxidase III in the Pectoralis superficialis muscle of 28-day-old quails fed diets containing 0, 8, and 12% glycerol. Total RNA was extracted (N = 10) and cDNA was amplified using specifics primers for qRT-PCR. Feed efficiency and feed intake were evaluated.

Analysis of the spatiotemporal expression of major genes in the TGF-β/Smad signaling pathway and correlation analysis using Hu sheep muscle tissue

Q. Z. Wang, Su, R., Lv, X. Y., Gao, W., Chen, L., Bao, J. J., Yu, J. R., Wang, L. H., Sun, W., Wang, Q. Z., Su, R., Lv, X. Y., Gao, W., Chen, L., Bao, J. J., Yu, J. R., Wang, L. H., and Sun, W., “Analysis of the spatiotemporal expression of major genes in the TGF-β/Smad signaling pathway and correlation analysis using Hu sheep muscle tissue”, vol. 15, p. -, 2016.

The mRNA expression levels of key genes (Smads, MSTN, and MyoG) in the TGF-β/Smad signaling pathway in Hu sheep at different growth stages (2 days, 2 months, and 6 months of age) and in different skeletal muscles (longissimus dorsi muscle and soleus muscle) and different genders were detected; and correlation of the Smad family (Smad2, Smad3, Smad4, and Smad7), MSTN, MyoG expressions was analyzed in Hu sheep.

Selection of reference genes in canine uterine tissues

M. Du, Wang, X., Yue, Y. W., Zhou, P. Y., Yao, W., Li, X., Ding, X. B., Liu, X. F., Guo, H., Ma, W. Z., Du, M., Wang, X., Yue, Y. W., Zhou, P. Y., Yao, W., Li, X., Ding, X. B., Liu, X. F., Guo, H., and Ma, W. Z., “Selection of reference genes in canine uterine tissues”, vol. 15, p. -, 2016.

Real-time quantitative polymerase chain reaction (RT-qPCR) is usually employed in gene expression studies in veterinary research, including in studies on canine pyometra. Canine pyometra is a common clinical disease in bitches. When using RT-qPCR, internal standards, such as reference genes, are necessary to investigate relative gene expression by quantitative measurements of mRNA levels. The aim of this study was to evaluate the stability of reference genes and select reference genes suitable for canine pyometra studies.

Transcriptomic differences of genes in the avian target of rapamycin (avTOR) pathway in a divergent line of meat-type chickens selected for feed efficiency

J. Lee, Aggrey, S. E., Lee, J., and Aggrey, S. E., “Transcriptomic differences of genes in the avian target of rapamycin (avTOR) pathway in a divergent line of meat-type chickens selected for feed efficiency”, vol. 15, p. -, 2016.

Avian target of rapamycin (avTOR) is a highly conserved serine-threonine kinase that serves as an intracellular energy and nutrient sensor and regulates cell division, growth, and apoptosis. The role of avTOR in mediating feed intake and growth in poultry is unknown. We studied avTOR signaling activities in duodenum and liver tissues at days 35 and 42 in chickens divergently selected for low (LRFI) or high (HRFI) residual feed intake. The differential expression of genes involved in the avTOR pathway was assayed using real-time polymerase chain reaction.

Sheep YAP1 temporal and spatial expression trend and its relation with MyHCs expression

W. Gao, Sun, W., Su, R., Lv, X. Y., Wang, Q. Z., Li, D., Musa, H. H., Chen, L., Zhou, H., Xu, H. S., Hua, W. H., Gao, W., Sun, W., Su, R., Lv, X. Y., Wang, Q. Z., Li, D., Musa, H. H., Chen, L., Zhou, H., Xu, H. S., Hua, W. H., Gao, W., Sun, W., Su, R., Lv, X. Y., Wang, Q. Z., Li, D., Musa, H. H., Chen, L., Zhou, H., Xu, H. S., and Hua, W. H., “Sheep YAP1 temporal and spatial expression trend and its relation with MyHCs expression”, vol. 15, p. -, 2016.

RT-PCR was used to study the temporal and spatial pattern of Yes-associated protein 1 (YAP1) and myosin heavy chain (MyHC) expression in four different skeletal muscles (i.e., longissimus dorsi muscle, soleus muscle, gastrocnemius muscle, and extensor digitorum longus) and three growth stages (i.e., 2 days old, 2 and 6 months old) of Hu Sheep.

Relationship between TLR4 and MCP2 expression levels and habitual abortion

X. P. Li, Song, L. N., Tian, L. P., Zhang, Y. S., Li, X. P., Song, L. N., Tian, L. P., Zhang, Y. S., Li, X. P., Song, L. N., Tian, L. P., and Zhang, Y. S., “Relationship between TLR4 and MCP2 expression levels and habitual abortion”, vol. 15, p. -, 2016.

Habitual abortion is associated with the altered expression of multiple genes. This study was carried out to investigate the relationship between expression of Toll-like receptor 4 (TLR4) and monocyte chemotactic protein 2 (MCP2 or CCL8) and habitual abortion. This was done by detecting and comparing their relative expression in peripheral blood and placental villi of patients and healthy fertile women.

Molecular characterization and expression analysis of the β-actin gene from the ridgetail white prawn Exopalaemon carinicauda

J. P. Liang, Wang, Y., Ge, Q. Q., Li, J. T., Liu, P., Li, J., Nie, G. X., Liang, J. P., Wang, Y., Ge, Q. Q., Li, J. T., Liu, P., Li, J., Nie, G. X., Liang, J. P., Wang, Y., Ge, Q. Q., Li, J. T., Liu, P., Li, J., and Nie, G. X., “Molecular characterization and expression analysis of the β-actin gene from the ridgetail white prawn Exopalaemon carinicauda”, vol. 15, p. -, 2016.

Actin is a highly conserved protein that is found in all eukaryotic cells, and has been widely used as an internal control gene in gene expression studies. In this study, we cloned an actin gene (named Ecβ-actin) from Exopalaemon carinicauda and determined its expression levels. The full-length cDNA of Ecβ-actin was 1335 bp long, comprising a 1131-bp ORF encoding 376 amino acids, a 65-bp 5'-UTR, and a 139-bp 3'-UTR with a poly(A) tail.

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