Gene expression

Optimal clone identifier for genomic shotgun libraries: "OC Identifier tool"

M. E. Cantão, Ferreira, J. E., and Lemos, E. G. M., Optimal clone identifier for genomic shotgun libraries: "OC Identifier tool", vol. 6, pp. 743-755, 2007.

In DNA microarray experiments, the gene fragments that are spotted on the slides are usually obtained by the synthesis of specific oligonucleotides that are able to amplify genes through PCR. Shotgun library sequences are an alternative to synthesis of primers for the study of each gene in the genome. The possibility of putting thousands of gene sequences into a single slide allows the use of shotgun clones in order to proceed with microarray analysis without a completely sequenced genome.

Control of gene expression and genetic manipulation in the Trypanosomatidae

S. M. R. Teixeira and da Rocha, W. D., Control of gene expression and genetic manipulation in the Trypanosomatidae, vol. 2. pp. 148-158, 2003.

Mechanisms controlling gene expression in trypanosomatids depend on several layers of regulation, with most regulatory pathways acting at a post-transcriptional level. Consequently, these parasites can follow the rapid changes associated with transitions between the insect vector and the mammalian host, with instant reprogramming of genetic expression.

Gene expression in Chromobacterium violaceum

R. Silva, Araripe, J. R., Rondinelli, E., and Ürményi, T. P., Gene expression in Chromobacterium violaceum, vol. 3, pp. 64-75, 2004.

The repertoire of 4,431 open reading frames (ORFs), eight rRNA operons and 98 tRNA genes of Chromobacterium violaceum must be expressed in a regulated manner for successful adaptation to a wide variety of environmental conditions. To accomplish this feat, the organism relies on protein machineries involved in transcription, RNA processing and translation. Analysis of the C.

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