Gene immunity

Antibody study in canine distemper virus nucleocapsid protein gene-immunized mice

B. Yuan, Li, X. Y., Zhu, T., Yuan, L., Hu, J. P., Chen, J., Gao, W., and Ren, W. Z., Antibody study in canine distemper virus nucleocapsid protein gene-immunized mice, vol. 14, pp. 3098-3105, 2015.

The gene for the nucleocapsid (N) protein of canine distemper virus was cloned into the pMD-18T vector, and positive recombinant plasmids were obtained by enzyme digestion and sequencing. After digestion by both EcoRI and KpnI, the plasmid was directionally cloned into the eukaryotic expression vector pcDNA; the positive clone pcDNA-N was screened by electrophoresis and then transfected into COS-7 cells. Immunofluorescence analysis results showed that the canine distemper virus N protein was expressed in the cytoplasm of transfected COS-7 cells.

Subscribe to Gene immunity