Gene transfection

Construction and identification of recombinant adenovirus carrying human TIMP-1shRNA gene

Y. L. Sun, Xie, H., Lin, H. L., Feng, Q., and Liu, Y., Construction and identification of recombinant adenovirus carrying human TIMP-1shRNA gene, vol. 14, pp. 199-208, 2015.

The aim of this study was to construct the recombinant adenovirus carrying human TIMP-1shRNA gene expression system for preliminary identification to lay the foundation for the further study of gene therapy.

Establishment of a rabbit model of coronary artery bypass graft and endothelial nitric oxide synthase gene transfection

Y. Zhu, Wang, H. - S., Li, X. - M., and Wang, Z. - W., Establishment of a rabbit model of coronary artery bypass graft and endothelial nitric oxide synthase gene transfection, vol. 14, pp. 1479-1486, 2015.

This study established an animal model of coronary artery bypass graft (CABG) surgery. The human endothelial nitric oxide synthase (eNOS) gene was transfected into grafted arterial walls to verify transfection efficiency. Forty rabbits were randomized into the following 4 equal groups: 1) eNOS gene transfection group (eNOS group); 2) empty eNOS gene transfection group (empty gene group); 3) control group; 4) normal femoral artery group. Grafted arteries, and normal carotid and femoral artery specimens were obtained 3 weeks later.

Effects of metallothionein-3 and metallothionein-1E gene transfection on proliferation, cell cycle, and apoptosis of esophageal cancer cells

Z. Q. Tian, Xu, Y. Z., Zhang, Y. F., Ma, G. F., He, M., and Wang, G. - Y., Effects of metallothionein-3 and metallothionein-1E gene transfection on proliferation, cell cycle, and apoptosis of esophageal cancer cells, vol. 12, pp. 4595-4603, 2013.

Metallothionein (MT)-3 has cell growth inhibitory activity, and is the only currently known MT subtype with unique physiological functions. The expression levels of MT-1E, a subtype of MT-1, were positively correlated with the degree of esophageal cancer malignancy. The present study aimed to investigate the effects of MT-3 and MT-1E gene transfection on the proliferation, cell cycle, and apoptosis of esophageal cancer cells. The cationic liposome method was used to transfect the esophageal cancer strains Eca-109 and TE13.

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