Genetic analysis

Body color development and genetic analysis of hybrid transparent crucian carp (Carassius auratus)

W. Xu, Tong, G. X., Geng, L. W., and Jiang, H. F., Body color development and genetic analysis of hybrid transparent crucian carp (Carassius auratus), vol. 14, pp. 4399-4407, 2015.

The aim of this study was to investigate the genetic mechanism of the transparent trait in transparent crucian carp. We observed body color development in transparent crucian carp larvae and analyzed heredity of color in hybrids produced with red crucian carp, ornamental carp, and red purse carp. The results showed that the body color of the newly hatched larvae matured into the adult pattern at approximately 54 days post-hatching.

Expression of genes associated with the biosynthetic pathways of abscisic acid, gibberellin, and ethylene during the germination of lettuce seeds

A. C. S. Clemente, Guimarães, R. M., Martins, D. C., Gomes, L. A. A., Caixeta, F., Reis, R. G. E., and Rosa, S. D. V. F., Expression of genes associated with the biosynthetic pathways of abscisic acid, gibberellin, and ethylene during the germination of lettuce seeds, vol. 14, pp. 4703-4715, 2015.

Seed germination and dormancy are complex phenomena that are controlled by many genes and environmental factors. Such genes are indicated by phytohormones that interact with each other, and may cause dormancy or promote seed germination. The objective of this study was to investigate gene expression associated with the biosynthetic pathways of abscisic acid (ABA), gibberellic acid (GA), and ethylene (ET) in dormant and germinated lettuce seeds.

Genetic analysis of the major gene plus polygene model in soybean resistance to Leguminivora glycinivorella

J. Zhang, Yao, D., Ma, J., Fu, Y. - P., Qu, J., and Wang, P. - W., Genetic analysis of the major gene plus polygene model in soybean resistance to Leguminivora glycinivorella, vol. 13, pp. 4983-4989, 2014.

In order to investigate the genetic characteristics of soybean Leguminivora glycinivorella resistance and to improve soybean resistance insectivorous breeding efficiency by applying the multi-generation joint analysis method of the major gene plus polygene model, 5 pedigrees and generations (P1, F1, P2, F2, and F2:3) were used as the materials to perform the soybean L. glycinivorella resistance multi-generation joint analysis. The results showed that soybean resistance to L.

Genetic analysis of differentiation of T-helper lymphocytes

Q. Wang, Li, M., Xia, L. C., Wen, G., Zu, H., and Gao, M., Genetic analysis of differentiation of T-helper lymphocytes, vol. 12, pp. 972-987, 2013.

In the human immune system, T-helper cells are able to differentiate into two lymphocyte subsets: Th1 and Th2. The intracellular signaling pathways of differentiation form a dynamic regulation network by secreting distinctive types of cytokines, while differentiation is regulated by two major gene loci: T-bet and GATA-3. We developed a system dynamics model to simulate the differentiation and re-differentiation process of T-helper cells, based on gene expression levels of T-bet and GATA-3 during differentiation of these cells.

Characterization and molecular mapping of a dwarf mutant in wheat

X. J. Jin, Sun, D. F., Li, H. Y., Yang, Y., and Sun, G. L., Characterization and molecular mapping of a dwarf mutant in wheat, vol. 12, pp. 3555-3565, 2013.

A spontaneous dwarf mutant of wheat was found in an F5 generation line derived from a cross between Huamai No. 9 and Een No. 1 in 1998; it was named Huaai 01. We characterized the genetic pattern of Huaai 01 and mapped the gene controlling the dwarf trait. This dwarf mutant was found insensitive to exogenous gibberellic acid treatment, based on the length of the first leaf and the coleoptile at the seedling stage, suggesting that it plays a crucial role in the gibberellin response pathway.

Utility of hair shafts from study skins for mitochondrial DNA analysis

N. Kurihara, Utility of hair shafts from study skins for mitochondrial DNA analysis, vol. 12, pp. 5396-5404, 2013.

The condition of mtDNA in hair shafts preserved in a museum was examined using 30 study skins of masked palm civets, Paguma larvata (Viverridae), collected between 1924 and 2011. Comparisons of extracts from fresh and burnt alum-fixed hair shafts showed that burnt alum, which is commonly used in taxidermy, had no harmful effect on the amount of total DNA and lengths of the mtDNA fragments.

RAPD assay of wild-type olives in Turkey

M. Sesli and Yegenoglu, E. D., RAPD assay of wild-type olives in Turkey, vol. 9, pp. 966-972, 2010.

Genetic similarities and distances between wild-type olives in Turkey were studied using an RAPD-PCR assay. Seven wild olive tree samples were collected from villages in Manisa and Izmir provinces. Genomic DNA was extracted from young leaves and the RAPD-PCR assay was used to generate RAPD markers. Sixty-five random primers obtained from Operon Technologies were tested for the assay (OP-A 1-20, OP-I 1-20, OP-Q 1-20, and OP-J 1-5). Thirty-two of these primers yielded 115 highly polymorphic bands. The mean number of usable bands per primer for all the samples was 3.59.

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