Genetic analysis

Genetic analyses of the major and minor locus groups of bacterial wilt resistance in tobacco using a diallel cross design

Y. L. Qian, Chen, J., Dong, J. J., Wu, Z. C., Liu, Y. H., Xue, B. Y., Shao, F. W., Sun, X. Y., Qian, Y. L., Chen, J., Dong, J. J., Wu, Z. C., Liu, Y. H., Xue, B. Y., Shao, F. W., Sun, X. Y., Qian, Y. L., Chen, J., Dong, J. J., Wu, Z. C., Liu, Y. H., Xue, B. Y., Shao, F. W., and Sun, X. Y., Genetic analyses of the major and minor locus groups of bacterial wilt resistance in tobacco using a diallel cross design, vol. 15, p. -, 2016.

Tobacco germplasm samples with various levels of resistance to bacterial wilt were selected to construct F1 combinations of parental inbred lines and orthogonal diallel crosses using samples collected in 2009 (15 germplasms), 2010 (15 germplasms), and 2011 (16 germplasms). A total of 1/2P (P + 1) experimental materials were used for analysis. Based on the analyses of major and minor locus groups, genetic effects on the incidence rate and index of bacterial wilt in tobacco were investigated on the 15th and 25th day during the early stage.

Sequence and phylogenetic analyses of the M and N genes of porcine epidemic diarrhea virus (PEDV) strains in Anhui Province, China

P. Sun, Gu, C. Y., Ding, Y. Y., Wei, J. H., Yin, Z. J., Geng, Z. Y., and Li, Y., Sequence and phylogenetic analyses of the M and N genes of porcine epidemic diarrhea virus (PEDV) strains in Anhui Province, China, vol. 14, pp. 13403-13413, 2015.

To assess the homology and phylogenetic relationship between porcine epidemic diarrhea virus (PEDV) Anhui strains and other PEDV strains, molecular homology and phylogenetic analyses of Anhui PEDV field strains were compared with those of reference strains. The results revealed that the M and N genes of PEDV were 681 and 1326 bp long, respectively. The nucleotide sequences of the N genes of Anhui PEDV strains were 95.9-99.9% homologous with each other, and the deduced amino acid sequences were 92.5-99.8% homologous.

Development of EST-SSR and TRAP markers from transcriptome sequencing data of the mango

C. Luo, Wu, H. X., Yao, Q. S., Wang, S. B., and Xu, W. T., Development of EST-SSR and TRAP markers from transcriptome sequencing data of the mango, vol. 14, pp. 7914-7919, 2015.

Mango is one of the most commercially important fruit crops in tropical and subtropical regions. To increase the efficiency of breeding strategies, two EST-derived marker systems were developed in the present study using information from the mango fruit transcriptome. Using simple sequence repeats, 218 of 230 primer pairs showed stable amplification for 7 mango genotypes with amplicons ranging from 84 to 160 bp; 93 of the primer pairs yielded polymorphic products. The proportion of polymorphic bands ranged from 16.67 to 100%, with a mean of 55.64%.

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